PUBLICATION
Newly identified Gon4l/Udu-interacting proteins implicate novel functions
- Authors
- Tsai, S.M., Chu, K.C., Jiang, Y.J.
- ID
- ZDB-PUB-200828-16
- Date
- 2020
- Source
- Scientific Reports 10: 14213 (Journal)
- Registered Authors
- Chu, Kuo-Chang, Jiang, Yun-Jin
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line
- Co-Repressor Proteins/metabolism*
- DNA (Cytosine-5-)-Methyltransferase 1/metabolism
- DNA-Binding Proteins/metabolism*
- Erythroid-Specific DNA-Binding Factors/metabolism*
- Mice
- RNA-Binding Proteins/metabolism
- Tumor Suppressor Proteins/metabolism
- Two-Hybrid System Techniques
- Ubiquitin Thiolesterase/metabolism
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 32848183 Full text @ Sci. Rep.
Citation
Tsai, S.M., Chu, K.C., Jiang, Y.J. (2020) Newly identified Gon4l/Udu-interacting proteins implicate novel functions. Scientific Reports. 10:14213.
Abstract
Mutations of the Gon4l/udu gene in different organisms give rise to diverse phenotypes. Although the effects of Gon4l/Udu in transcriptional regulation have been demonstrated, they cannot solely explain the observed characteristics among species. To further understand the function of Gon4l/Udu, we used yeast two-hybrid (Y2H) screening to identify interacting proteins in zebrafish and mouse systems, confirmed the interactions by co-immunoprecipitation assay, and found four novel Gon4l-interacting proteins: BRCA1 associated protein-1 (Bap1), DNA methyltransferase 1 (Dnmt1), Tho complex 1 (Thoc1, also known as Tho1 or HPR1), and Cryptochrome circadian regulator 3a (Cry3a). Furthermore, all known Gon4l/Udu-interacting proteins-as found in this study, in previous reports, and in online resources-were investigated by Phenotype Enrichment Analysis. The most enriched phenotypes identified include increased embryonic tissue cell apoptosis, embryonic lethality, increased T cell derived lymphoma incidence, decreased cell proliferation, chromosome instability, and abnormal dopamine level, characteristics that largely resemble those observed in reported Gon4l/udu mutant animals. Similar to the expression pattern of udu, those of bap1, dnmt1, thoc1, and cry3a are also found in the brain region and other tissues. Thus, these findings indicate novel mechanisms of Gon4l/Udu in regulating CpG methylation, histone expression/modification, DNA repair/genomic stability, and RNA binding/processing/export.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping