PUBLICATION
Tetrabromobisphenol A perturbs erythropoiesis and impairs blood circulation in zebrafish embryos
- Authors
- Pang, S., Gao, Y., Li, A., Yao, X., Qu, G., Hu, L., Liang, Y., Song, M., Jiang, G.
- ID
- ZDB-PUB-200826-8
- Date
- 2020
- Source
- Environmental science & technology 54(20): 12998-13007 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Erythropoiesis
- Flame Retardants*
- Polybrominated Biphenyls*/toxicity
- Zebrafish
- PubMed
- 32841016 Full text @ Env. Sci. Tech.
Citation
Pang, S., Gao, Y., Li, A., Yao, X., Qu, G., Hu, L., Liang, Y., Song, M., Jiang, G. (2020) Tetrabromobisphenol A perturbs erythropoiesis and impairs blood circulation in zebrafish embryos. Environmental science & technology. 54(20):12998-13007.
Abstract
Tetrabromobisphenol A (TBBPA), a ubiquitous environmental pollutant, has been implicated in developmental toxicity of aquatic animals. However, the impact of TBBPA on development and the related mechanism have not been fully elucidated. In this study, using a live imaging technique and transgenic labeling of zebrafish embryos, we described the toxic effects of TBBPA on hematopoietic development in zebrafish. We demonstrated that TBBPA induced erythroid precursor expansion in the intermediate cell mass (ICM), which perturbed the onset of blood circulation at 24-26 hours post fertilization (hpf). Consequently, excessive blood cells accumulated in the posterior blood island (PBI) and vascular cells formed defective caudal veins (CVs), preventing blood cell flow to the heart at 32-34 hpf. We found that the 1-cell to 50% epiboly stage was the most sensitive period to TBBPA exposure during hematopoietic development. Furthermore, our results demonstrated that PBI malformation induced by TBBPA resulted from effects on erythroid precursor cells which might involve THR signaling in complex ways. These findings will improve the understanding of TBBPA-induced developmental toxicity in teleost.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping