PUBLICATION
The long non-coding RNA landscape of cardiac regeneration in zebrafish
- Authors
- Lumley, A.I., Zhang, L., Ernens, I., Leszek, P., Devaux, Y.
- ID
- ZDB-PUB-200825-14
- Date
- 2020
- Source
- The Canadian journal of cardiology 37(3): 484-492 (Journal)
- Registered Authors
- Keywords
- cardiac regeneration, heart failure, lncRNA, zebrafish
- Datasets
- GEO:GSE120962
- MeSH Terms
-
- RNA, Long Noncoding/genetics*
- RNA, Long Noncoding/metabolism
- Regeneration/physiology*
- Animals
- Disease Models, Animal
- Heart Failure/genetics*
- Heart Failure/metabolism
- Heart Failure/physiopathology
- Gene Expression Regulation*
- Zebrafish/genetics
- PubMed
- 32835855 Full text @ Can J Cardiol
Citation
Lumley, A.I., Zhang, L., Ernens, I., Leszek, P., Devaux, Y. (2020) The long non-coding RNA landscape of cardiac regeneration in zebrafish. The Canadian journal of cardiology. 37(3):484-492.
Abstract
Background Novel therapeutic targets of heart failure (HF) are needed. Long non-coding RNAs (lncRNAs) are engaged during cardiac regeneration. Unlike in humans, zebrafish naturally undergo cardiac regeneration following HF. We aimed to describe the landscape of lncRNAs during regeneration in a zebrafish model of HF and to investigate their human homolog.
Methods HF was established in adult zebrafish through tri-weekly incubations with an anemia inducing drug - phenylhydrazine hydrochloride (PHZ). After 5 weeks, PHZ treatment ceased and fish were followed through a regeneration period of 14 days. Total RNA was extracted from the hearts of adult zebrafish after establishment of HF and at 2, 5 and 9 days after treatment cessation (9 hearts per condition at each timepoint). Gene regulation patterns were characterized using bioinformatics and validated by quantitative PCR.
Results We obtained 14,340 lncRNAs from re-annotated Affymetrix zebrafish microarray. Of these, 187 lncRNAs were found differentially expressed (false discovery rate < 0.05 and fold change ≥ 2) at at least one time point. 85% of differentially expressed lncRNAs overlapped or were close to (distance < 10kb) protein-coding genes which were mostly related to muscle development in Gene Ontology analyses. 57 lncRNAs had human homologs according to orientation relative to their conserved protein coding neighbours.
Conclusions LncRNAs are differentially expressed during regeneration following HF in adult zebrafish and could be potential future therapeutic targets. The extent to which lncRNAs contribute to cardiac regeneration is a worthy avenue for future research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping