PUBLICATION
Kinesin-7 CENP-E regulates cell division, gastrulation and organogenesis in development
- Authors
- Yu, K.W., She, Z.Y., Wei, Y.L., Zhong, N.
- ID
- ZDB-PUB-200818-10
- Date
- 2020
- Source
- European journal of cell biology 99: 151107 (Journal)
- Registered Authors
- Keywords
- CENP-E, Cell division, Chromosome alignment, Genesis, Kinesin-7, Mitosis, Organ
- MeSH Terms
-
- Animals
- Cell Division/physiology*
- Chromosomal Proteins, Non-Histone/metabolism*
- Humans
- Kinesins/metabolism*
- Organogenesis/physiology*
- Zebrafish
- PubMed
- 32800279 Full text @ Eur. J. Cell Biol.
Citation
Yu, K.W., She, Z.Y., Wei, Y.L., Zhong, N. (2020) Kinesin-7 CENP-E regulates cell division, gastrulation and organogenesis in development. European journal of cell biology. 99:151107.
Abstract
Kinesin-7 CENP-E motor protein is essential for chromosome alignment and kinetochore-microtubule attachment in cell division. Human CENP-E has recently identified to be linked with the microcephalic primordial dwarfism syndromes associated with a smaller head, brain malformations and a prominent nose. However, the roles of CENP-E in embryonic development remain largely unknown. In this study, we find that zebrafish CENP-E inhibition results in defects in early zygote cleavage, including asymmetric cell division, cell cycle arrest and the developmental abnormalities. We also demonstrate that CENP-E ablation in cultured cells leads to chromosome misalignment, spindle abnormalities and interruptions of the cell cycle. These observations suggest that CENP-E plays a key role in early cell division and cell cycle progression. Furthermore, we also find that CENP-E inhibition results in the defects in the epiboly, the developmental arrest, the smaller head and the abnormal embryo during zebrafish embryogenesis. Our data demonstrate new functions of CENP-E in development and provide insights into its essential roles in organogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping