PUBLICATION

Systematic profiling of early regulators during tissue regeneration using zebrafish model

Authors
Shi, L., Chen, C., Yin, Z., Wei, G., Xie, G., Liu, D.
ID
ZDB-PUB-200811-8
Date
2020
Source
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society   29(1): 189-195 (Journal)
Registered Authors
Liu, Dong
Keywords
none
MeSH Terms
  • Amputation
  • Animal Fins/physiology*
  • Animal Fins/surgery
  • Animals
  • Disease Models, Animal
  • Fibroblast Growth Factors/biosynthesis
  • Fibroblast Growth Factors/genetics*
  • Gene Expression Regulation*
  • RNA/genetics
  • Regeneration/genetics*
  • Signal Transduction
  • Surgical Wound/genetics*
  • Surgical Wound/metabolism
  • Surgical Wound/pathology
  • Wound Healing/genetics*
  • Zebrafish/genetics*
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics*
PubMed
32776615 Full text @ Wound Repair Regen.
Abstract
Great progresses have been made in comprehension of tissue regeneration process. However, one of the central questions in regeneration research remains to be deciphered is what factors initiate regenerative process. In present study, we focused on systematic profiling of early regulators in tissue regeneration via high-throughput screening on zebrafish caudal fin model. Firstly, 53 GO-annotated regeneration-related genes, which were specifically activated upon fin amputation, were identified according to the transcriptomic analysis. Moreover, qRT-PCR analysis of a couple of randomly selected genes from the aforementioned gene list validated our sequencing results. These studies confirmed the reliability of transcriptome sequencing analysis. Fibroblast growth factor 20a (fgf20a) is a key initial factor in the regeneration of zebrafish. Through a gene expression correlation analysis, we discovered a collection of 70 genes correlating with fgf20a, whose expression increased promptly at 2 days post amputation (dpa) and went down to the basal level until the completion of fin regeneration. In addition, two genes, socs3b and nppc, were chosen to investigate their functions during the fin regeneration. Inhibition of either of those genes significantly delayed the regenerative process. Taken together, we provided a simple and effective time-saving strategy that may serve as a tool for identifying early regulators in regeneration and identified 71 genes as early regulators of fin regeneration.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes