PUBLICATION
In vivo assessment of the toxicity of electronic cigarettes to zebrafish (Danio rerio) embryos, following gestational exposure, in terms of mortality, developmental toxicity, and hair cell damage: Toxicity of E-cigs to zebrafish embryos
- Authors
- Chang, Y.S., Park, S.M., Rah, Y.C., Han, E.J., Koun, S.I., Chang, J., Choi, J.
- ID
- ZDB-PUB-200811-4
- Date
- 2020
- Source
- Human & Experimental Toxicology 40(1): 148-157 (Journal)
- Registered Authors
- Keywords
- Electronic cigarettes, embryonic development, hair cell, toxicity, zebrafish
- MeSH Terms
-
- Animals
- Electronic Nicotine Delivery Systems*
- Embryo, Nonmammalian/drug effects*
- Hair Cells, Auditory
- Humans
- Smoking Cessation
- Tobacco Products
- Tobacco Use Cessation Devices
- Zebrafish/embryology
- PubMed
- 32772711 Full text @ Hum. Exp. Toxicol.
Citation
Chang, Y.S., Park, S.M., Rah, Y.C., Han, E.J., Koun, S.I., Chang, J., Choi, J. (2020) In vivo assessment of the toxicity of electronic cigarettes to zebrafish (Danio rerio) embryos, following gestational exposure, in terms of mortality, developmental toxicity, and hair cell damage: Toxicity of E-cigs to zebrafish embryos. Human & Experimental Toxicology. 40(1):148-157.
Abstract
With the ban of conventional cigarettes from public spaces, electronic cigarette (E-cig) liquids have emerged as a nicotine replacement treatment for smoking cessation. However, consumers possess little knowledge of the ingredients and health effects of E-cig liquids following exposure. This study evaluated hair cell damage and developmental toxicities following gestational exposure to E-cig liquids. Zebrafish embryos were exposed to E-cig liquids at different concentrations (0.1%, 0.2%, and 0.4%). Embryonic developmental toxicity and hair cell damage was evaluated at 6 and 7 d, respectively, after fertilization. The average number of hair cells in the anterior lateral line (ALL) and posterior lateral line (PLL) following E-cig exposure was compared to that of the control. Morphological abnormalities and heart rate were evaluated. E-cig liquids significantly damaged the hair cells in the ALL, compared to the control (control; 52.85 ± 5.29 cells, 0.1% E-cig; 49.43 ± 7.70 cells, 0.2% E-cig; 40.68 ± 12.00 cells, 0.4% E-cig; 32.14 ± 20.75%; n = 29-40; p < 0.01). At high concentrations, E-cig liquids significantly damaged the hair cells in the PLL (control; 36.88 ± 5.43 cells, 0.1% E-cig; 33.06 ± 5.21 cells, 0.2% E-cig; 30.95 ± 8.03 cells, 0.4% E-cig; 23.72 ± 15.53%, n = 29-40; p < 0.01). No morphological abnormalities in body shape, somites, notochord, tail, and pectoral fin were observed; however, abnormalities were observed in the dorsal fin and heart rate at high concentrations. Thus, gestational exposure to E-cigs significantly damaged hair cells in a concentration-dependent manner and induced developmental toxicities to the dorsal fin and heart rate at high concentrations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping