PUBLICATION
Chloroquine kills hair cells in zebrafish lateral line and murine cochlear cultures: Implications for ototoxicity
- Authors
- Davis, S.N., Wu, P., Camci, E.D., Simon, J.A., Rubel, E.W., Raible, D.W.
- ID
- ZDB-PUB-200810-35
- Date
- 2020
- Source
- Hearing Research 395: 108019 (Journal)
- Registered Authors
- Keywords
- Anti-malarial, Hearing loss, Ototoxicity, Vestibular loss
- MeSH Terms
-
- Ototoxicity
- Cell Survival/drug effects*
- Antiviral Agents/toxicity
- Chloroquine/analogs & derivatives*
- Chloroquine/toxicity
- Zebrafish
- Cells, Cultured
- Models, Animal
- Hydroxychloroquine/toxicity*
- Mice
- Lateral Line System/drug effects*
- Animals
- Hair Cells, Auditory/cytology
- Hair Cells, Auditory/drug effects*
- Larva/drug effects
- PubMed
- 32768772 Full text @ Hear. Res.
Citation
Davis, S.N., Wu, P., Camci, E.D., Simon, J.A., Rubel, E.W., Raible, D.W. (2020) Chloroquine kills hair cells in zebrafish lateral line and murine cochlear cultures: Implications for ototoxicity. Hearing Research. 395:108019.
Abstract
Hearing and balance deficits have been reported during and following treatment with the antimalarial drug chloroquine. However, experimental work examining the direct actions of chloroquine on mechanoreceptive hair cells in common experimental models is lacking. This study examines the effects of chloroquine on hair cells using two common experimental models: the zebrafish lateral line and neonatal mouse cochlear cultures. Zebrafish larvae were exposed to varying concentrations of chloroquine phosphate or hydroxychloroquine for 1 h or 24 h, and hair cells assessed by antibody staining. A significant, dose-dependent reduction in the number of surviving hair cells was seen across conditions for both exposure periods. Hydroxychloroquine showed similar toxicity. In mouse cochlear cultures, chloroquine damage was specific to outer hair cells in tissue from the cochlear basal turn, consistent with susceptibility to other ototoxic agents. These findings suggest a need for future studies employing hearing and balance monitoring during exposure to chloroquine and related compounds, particularly with interest in these compounds as therapeutics against viral infections including coronavirus.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping