PUBLICATION
Melatonin suppresses both osteoblast and osteoclast differentiation through repression of epidermal Erk signaling in the zebrafish scale
- Authors
- Kobayashi-Sun, J., Suzuki, N., Hattori, A., Yamaguchi, M., Kobayashi, I.
- ID
- ZDB-PUB-200810-29
- Date
- 2020
- Source
- Biochemical and Biophysical Research Communications 530(4): 644-650 (Journal)
- Registered Authors
- Kobayashi, Isao
- Keywords
- Melatonin, Osteoblasts, Osteoclasts, Scale, Zebrafish
- MeSH Terms
-
- Animal Scales/cytology
- Animal Scales/drug effects
- Animal Scales/physiology
- Animals
- Cell Differentiation/drug effects
- Disease Models, Animal
- Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors*
- Extracellular Signal-Regulated MAP Kinases/metabolism
- Fracture Healing/drug effects
- MAP Kinase Signaling System/drug effects*
- Melatonin/pharmacology*
- Osteoblasts/cytology
- Osteoblasts/drug effects*
- Osteoclasts/cytology
- Osteoclasts/drug effects*
- Osteogenesis/drug effects*
- Zebrafish/physiology
- PubMed
- 32768192 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kobayashi-Sun, J., Suzuki, N., Hattori, A., Yamaguchi, M., Kobayashi, I. (2020) Melatonin suppresses both osteoblast and osteoclast differentiation through repression of epidermal Erk signaling in the zebrafish scale. Biochemical and Biophysical Research Communications. 530(4):644-650.
Abstract
Melatonin has been implicated in the regulation of bone metabolism; however, the molecular mechanisms underlying its involvement in fracture healing are still obscure. We previously developed an in vivo fracture healing model using the scale of a double-transgenic zebrafish, trap:GFP; osterix:mCherry, which labels osteoclasts and osteoblasts with GFP and mCherry, respectively. Here we show using this model that melatonin inhibits both osteoblast and osteoclast differentiation under fracture stress through the repression of Erk signaling in epidermal cells of the scale. Melatonin treatment resulted in reduced numbers of both osteoblasts and osteoclasts in the fractured scale. Immunochemistry analysis revealed that Erk signals in epidermal cells, which express melatonin receptors, were greatly enhanced in response to fracture stress, but this enhancement was blocked by melatonin treatment. Moreover, inhibition of Erk signaling phenocopied the effects of melatonin treatment in the fractured scale. Collectively, these data suggest that the activation of epidermal Erk signaling is required for both osteoblast and osteoclast differentiation in the early stage of fracture healing, and melatonin suppresses epidermal Erk signaling, leading to impaired fracture healing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping