PUBLICATION
Characterization of monoaminergic neurochemicals in the different brain regions of adult zebrafish
- Authors
- Mayol-Cabré, M., Prats, E., Raldúa, D., Gómez-Canela, C.
- ID
- ZDB-PUB-200810-2
- Date
- 2020
- Source
- The Science of the total environment 745: 141205 (Journal)
- Registered Authors
- Raldúa, Demetrio
- Keywords
- Adult zebrafish, Brain, Liquid chromatography, Neurotransmitters, Tandem mass spectrometry
- MeSH Terms
-
- Animals
- Brain
- Chromatography, High Pressure Liquid
- Chromatography, Liquid
- Neurotransmitter Agents
- Reproducibility of Results
- Tandem Mass Spectrometry*
- Zebrafish*
- PubMed
- 32758735 Full text @ Sci. Total Environ.
Citation
Mayol-Cabré, M., Prats, E., Raldúa, D., Gómez-Canela, C. (2020) Characterization of monoaminergic neurochemicals in the different brain regions of adult zebrafish. The Science of the total environment. 745:141205.
Abstract
Monoaminergic neurotransmitters are the main components that regulate of a lot of processes in the vertebrate brain. There is growing interest to monitor the changes produced in these neurochemicals due to the large number of exogenous agents, such as pharmaceuticals and drugs of abuse, targeting and affecting this system. Adult zebrafish (Danio rerio) shares the common neurotransmitter pathways and nervous system organization with mammals. Therefore, a method based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been developed for the first time to study the profile of ten monoaminergic neurochemicals in the anterior, middle and posterior brain regions of adult zebrafish. Moreover, the applied LC-MS/MS method has been studied in terms of quality such as linearity, sensitivity and intra- and inter-day precision. The analytical method based in LC-MS/MS has become a new source in neurotoxicology using adult zebrafish as research model. Significant differences on the levels of these neurotransmitters have been found between the different brain regions. CAPSULE: The profile of ten monoaminergic neurochemicals in the main three brain areas of adult zebrafish has been reported for the first time in this manuscript.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping