Folic acid reduces the ethanol-induced morphological and behavioral defects in embryonic and larval zebrafish (Danio rerio) as a model for fetal alcohol spectrum disorder (FASD)
- Cadena, P.G., Cadena, M.R.S., Sarmah, S., Marrs, J.A.
- Reproductive toxicology (Elmsford, N.Y.) 96: 249-257 (Journal)
- Registered Authors
- Marrs, James A.
- Fetal alcohol spectrum disorder, Optomotor response, Sleep, Swimming activity, Teratogenicity, Thigmotaxis, Zebrafish
- MeSH Terms
- Abnormalities, Drug-Induced/drug therapy*
- Abnormalities, Drug-Induced/embryology
- Air Sacs/abnormalities
- Behavior, Animal/drug effects
- Disease Models, Animal*
- Embryo, Nonmammalian
- Eye Abnormalities/chemically induced
- Fetal Alcohol Spectrum Disorders*
- Folic Acid/pharmacology*
- Locomotion/drug effects
- Sleep/drug effects
- Yolk Sac/abnormalities
- 32763456 Full text @ Reprod. Toxicol.
Cadena, P.G., Cadena, M.R.S., Sarmah, S., Marrs, J.A. (2020) Folic acid reduces the ethanol-induced morphological and behavioral defects in embryonic and larval zebrafish (Danio rerio) as a model for fetal alcohol spectrum disorder (FASD). Reproductive toxicology (Elmsford, N.Y.). 96:249-257.
The objective of this work was to determine whether folic acid (FA) reduces the embryonic ethanol (EtOH) exposure induced behavioral and morphological defects in our zebrafish fetal alcohol spectrum disorder (FASD) model. Teratogenic effects, mortality, the excitatory light-dark locomotion (ELD), sleep (SL), thigmotaxis (TH), touch sensitivity (TS), and optomotor response (OMR) tests were evaluated in larvae (6-7 days post-fertilization) using four treatment conditions: Untreated, FA, EtOH and EtOH + FA. FA reduced morphological defects on heart, eyes and swim bladder inflation seen in EtOH exposed fish. The larvae were more active in the dark than in light conditions, and EtOH reduced the swimming activity in the ELD test. EtOH affected the sleep pattern, inducing several arousal periods and increasing inactivity in zebrafish. FA reduces these toxic effects and produced more consistent inactivity during the night, reducing the arousal periods. FA also prevented the EtOH-induced defects in thigmotaxis and optomotor response of the larvae. We conclude that in this FASD model, EtOH exposure produced several teratogenic and behavioral defects, FA reduced, but did not totally prevent, these defects. Understanding of EtOH-induced behavioral defects could help to identify new therapeutic or prevention strategies for FASD.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes