PUBLICATION
Genetic Engineering of Zebrafish in Cancer Research
- Authors
- Raby, L., Völkel, P., Le Bourhis, X., Angrand, P.O.
- ID
- ZDB-PUB-200810-10
- Date
- 2020
- Source
- Cancers 12(8): (Review)
- Registered Authors
- Angrand, Pierre-Olivier, Raby, Ludivine, Völkel, Pamela
- Keywords
- TILLING, cancer model, chemical carcinogenesis, genetic screens, genome editing, transgenesis, zebrafish
- MeSH Terms
- none
- PubMed
- 32759814 Full text @ Cancers
Citation
Raby, L., Völkel, P., Le Bourhis, X., Angrand, P.O. (2020) Genetic Engineering of Zebrafish in Cancer Research. Cancers. 12(8).
Abstract
Zebrafish (Danio rerio) is an excellent model to study a wide diversity of human cancers. In this review, we provide an overview of the genetic and reverse genetic toolbox allowing the generation of zebrafish lines that develop tumors. The large spectrum of genetic tools enables the engineering of zebrafish lines harboring precise genetic alterations found in human patients, the generation of zebrafish carrying somatic or germline inheritable mutations or zebrafish showing conditional expression of the oncogenic mutations. Comparative transcriptomics demonstrate that many of the zebrafish tumors share molecular signatures similar to those found in human cancers. Thus, zebrafish cancer models provide a unique in vivo platform to investigate cancer initiation and progression at the molecular and cellular levels, to identify novel genes involved in tumorigenesis as well as to contemplate new therapeutic strategies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping