PUBLICATION
Are microRNAs responsible for cardiac hypertrophy in fish and mammals? What we can learn in the activation process in a zebrafish ex vivo model
- Authors
- Romano, N., Ceci, M.
- ID
- ZDB-PUB-200719-19
- Date
- 2020
- Source
- Biochimica et biophysica acta. Molecular basis of disease 1866(11): 165896 (Journal)
- Registered Authors
- Keywords
- Cardiac hypertrophy, HSP70, Phenylephrine, WT1, Zebrafish, miR 1, miR 133a
- MeSH Terms
-
- Animals
- Cardiomegaly/metabolism*
- Cardiomegaly/pathology
- Disease Models, Animal
- HSP70 Heat-Shock Proteins/metabolism
- Hypertrophy/metabolism*
- Hypertrophy/pathology
- Mammals
- MicroRNAs/metabolism*
- Pericardium/drug effects
- Pericardium/metabolism*
- Pericardium/pathology
- Phenylephrine/pharmacology
- Zebrafish/metabolism*
- PubMed
- 32681863 Full text @ BBA Molecular Basis of Disease
Citation
Romano, N., Ceci, M. (2020) Are microRNAs responsible for cardiac hypertrophy in fish and mammals? What we can learn in the activation process in a zebrafish ex vivo model. Biochimica et biophysica acta. Molecular basis of disease. 1866(11):165896.
Abstract
Recent studies have correlated dysregulated miRNA expression with diseased hearts. With the aim of developing an easily manipulated experimental model, phenylephrine (PE) was added to cultured zebrafish hearts to study the expression of miR1 and miR133a by qRT-PCR. Both miRs were downregulated, with greater downregulation leading to higher hypertrophy. The involvement of this miRs was confirmed by the in-vivo inoculation of complementary sequences (AmiR1 and AmiR133a). HSP70 (involved in transporting proteins and in anti-apoptosis processes) was increased in both treatments. Hyperplasia was observed in the epicardium based on WT1 expression (embryonic epicardial cell marker) in both the PE treatment and AmiR133a treatment. The treatment with AmiR1 showed only cardiomyocyte hypertrophy. This ex-vivo model revealed that miR1 and miR133a play a key role in activating early processes leading to myocardium hypertrophy and epicardium hyperplasia and confirmed the expected similarities with hypertrophic disease that occurs in humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping