PUBLICATION
Functional and behavioral signatures of Kv7 activator drug subtypes
- Authors
- Kanyo, R., Wang, C.K., Locskai, L.F., Li, J., Allison, W.T., Kurata, H.T.
- ID
- ZDB-PUB-200712-10
- Date
- 2020
- Source
- Epilepsia 61(8): 1678-1690 (Journal)
- Registered Authors
- Allison, Ted
- Keywords
- CaMPARI, Kv7 channel activator, epilepsy, potassium channel, retigabine, zebrafish
- MeSH Terms
-
- Anilides/pharmacology*
- Animals
- Animals, Genetically Modified
- Anticonvulsants/classification
- Anticonvulsants/pharmacology*
- Bridged Bicyclo Compounds/pharmacology*
- Calcium/metabolism*
- Carbamates/pharmacology*
- Disease Models, Animal
- Drug Resistance/genetics
- Epilepsy/drug therapy*
- Epilepsy/metabolism
- In Vitro Techniques
- KCNQ Potassium Channels/drug effects*
- KCNQ Potassium Channels/genetics
- KCNQ Potassium Channels/metabolism
- KCNQ2 Potassium Channel/drug effects
- KCNQ2 Potassium Channel/genetics
- KCNQ2 Potassium Channel/metabolism
- KCNQ3 Potassium Channel/drug effects
- KCNQ3 Potassium Channel/genetics
- KCNQ3 Potassium Channel/metabolism
- Luminescent Proteins/genetics
- Membrane Potentials
- Mutation
- Neurons/drug effects*
- Neurons/metabolism
- Optical Imaging
- Patch-Clamp Techniques
- Phenylenediamines/pharmacology*
- Seizures/drug therapy*
- Seizures/metabolism
- Zebrafish
- PubMed
- 32652600 Full text @ Epilepsia
Citation
Kanyo, R., Wang, C.K., Locskai, L.F., Li, J., Allison, W.T., Kurata, H.T. (2020) Functional and behavioral signatures of Kv7 activator drug subtypes. Epilepsia. 61(8):1678-1690.
Abstract
Objective Voltage-gated potassium channels of the KCNQ (Kv7) family are targeted by a variety of activator compounds with therapeutic potential for treatment of epilepsy. Exploration of this drug class has revealed a variety of effective compounds with diverse mechanisms. In this study, we aimed to clarify functional criteria for categorization of Kv7 activator compounds, and to compare the effects of prototypical drugs in a zebrafish larvae model.
Methods In vitro electrophysiological approaches with recombinant ion channels were used to highlight functional properties important for classification of drug mechanisms. We also benchmarked the effects of representative antiepileptic Kv7 activator drugs using behavioral seizure assays of zebrafish larvae and in vivo Ca2+ imaging with the ratiometric Ca2+ sensor CaMPARI.
Results Drug effects on channel gating kinetics, and drug sensitivity profiles to diagnostic channel mutations, were used to highlight properties for categorization of Kv7 activator drugs into voltage sensor-targeted or pore-targeted subtypes. Quantifying seizures and ratiometric Ca2+ imaging in freely swimming zebrafish larvae demonstrated that while all Kv7 activators tested lead to suppression of neuronal excitability, pore-targeted activators (like ML213 and retigabine) strongly suppress seizure behavior, whereas ICA-069673 triggers a seizure-like hypermotile behavior.
Significance This study suggests criteria to categorize antiepileptic Kv7 activator drugs based on their underlying mechanism. We also establish the use of in vivo CaMPARI as a tool for screening effects of anticonvulsant drugs on neuronal excitability in zebrafish. In summary, despite a shared ability to suppress neuronal excitability, our findings illustrate how mechanistic differences between Kv7 activator subtypes influence their effects on heteromeric channels and lead to vastly different in vivo outcomes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping