PUBLICATION
Efficacy of Moxifloxacin against Mycobacterium abscessus in Zebrafish Model in vivo
- Authors
- Nie, W.J., Xie, Z.Y., Gao, S., Teng, T.L., Zhou, W.Q., Shang, Y.Y., Jing, W., Shi, W.H., Wang, Q.F., Huang, X.R., Cai, B.Y., Wang, J., Wang, J., Guo, R., Ge, Q.P., Nie, L.H., Han, X.Q., Du, Y.D., Chu, N.H.
- ID
- ZDB-PUB-200620-5
- Date
- 2020
- Source
- Biomedical and environmental sciences : BES 33: 350-358 (Journal)
- Registered Authors
- Keywords
- Azithromycin, Moxifloxacin, Mycobacterium abscessus, Zebrafish
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents/pharmacology*
- Disease Models, Animal
- Moxifloxacin/pharmacology*
- Mycobacterium Infections, Nontuberculous/drug therapy*
- Mycobacterium abscessus/drug effects*
- Zebrafish*
- PubMed
- 32553079 Full text @ Biomed. Environ. Sci.
Citation
Nie, W.J., Xie, Z.Y., Gao, S., Teng, T.L., Zhou, W.Q., Shang, Y.Y., Jing, W., Shi, W.H., Wang, Q.F., Huang, X.R., Cai, B.Y., Wang, J., Wang, J., Guo, R., Ge, Q.P., Nie, L.H., Han, X.Q., Du, Y.D., Chu, N.H. (2020) Efficacy of Moxifloxacin against Mycobacterium abscessus in Zebrafish Model in vivo. Biomedical and environmental sciences : BES. 33:350-358.
Abstract
Objective Moxifloxacin (MFX) shows good in vitro activity against Mycobacterium abscessus and can be a possible antibiotic therapy to treat M. abscessus infection; however, other studies have shown a lower or no activity. We aimed to evaluate MFX activity against M. abscessus using zebrafish (ZF) model in vivo.
Methods A formulation of M. abscessus labeled with CM-Dil was micro-injected into ZF. Survival curves were determined by recording dead ZF every day. ZF were lysed, and colony-forming units (CFUs) were enumerated. Bacteria dissemination and fluorescence intensity in ZF were analyzed. Inhibition rates of MFX and azithromycin (AZM, positive control) were determined and compared.
Results Significantly increased survival rate was observed with different AZM concentrations. However, increasing MFX concentration did not result in a significant decrease in ZF survival curve. No significant differences in bacterial burdens by CFU loads were observed between AZM and MFX groups at various concentrations. Bacterial fluorescence intensity in ZF was significantly correlated with AZM concentration. However, with increasing MFX concentration, fluorescence intensity decreased slightly when observed under fluorescence microscope. Transferring rates at various concentrations were comparable between the MFX and AZM groups, with no significant difference.
Conclusion MFX showed limited efficacy against M. abscessusin vivo using ZF model. Its activity in vivo needs to be confirmed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping