PUBLICATION
Activation of Angiogenesis and Wound Healing in Diabetic Mice Using NO-Delivery Dinitrosyl Iron Complexes
- Authors
- Chen, Y.J., Wu, S.C., Wang, H.C., Wu, T.H., Yuan, S.F., Lu, T.T., Liaw, W.F., Wang, Y.M.
- ID
- ZDB-PUB-200618-13
- Date
- 2019
- Source
- Molecular pharmaceutics 16: 4241-4251 (Journal)
- Registered Authors
- Keywords
- angiogenesis, growth factors, nitric oxide, wound healing
- MeSH Terms
-
- Zebrafish
- Mice
- Diabetes Mellitus, Experimental/drug therapy*
- Diabetes Mellitus, Experimental/metabolism
- Diabetes Mellitus, Experimental/pathology
- Mice, Inbred BALB C
- Neovascularization, Pathologic/metabolism
- Neovascularization, Pathologic/pathology
- Neovascularization, Pathologic/prevention & control*
- Wound Healing/drug effects*
- Drug Delivery Systems*
- Wounds and Injuries/pathology
- Wounds and Injuries/prevention & control*
- Cell Survival
- Chorioallantoic Membrane/drug effects
- Cells, Cultured
- Animals
- Vascular Endothelial Growth Factor A/metabolism
- Chick Embryo
- Humans
- Iron/administration & dosage*
- Hindlimb
- Endothelium, Vascular/cytology
- Endothelium, Vascular/drug effects
- Endothelium, Vascular/metabolism
- Ischemia/pathology
- Ischemia/prevention & control
- Nitric Oxide/chemistry
- Nitric Oxide/metabolism*
- Nitrogen Oxides/administration & dosage*
- Female
- PubMed
- 31436106 Full text @ Mol. Pharm.
Citation
Chen, Y.J., Wu, S.C., Wang, H.C., Wu, T.H., Yuan, S.F., Lu, T.T., Liaw, W.F., Wang, Y.M. (2019) Activation of Angiogenesis and Wound Healing in Diabetic Mice Using NO-Delivery Dinitrosyl Iron Complexes. Molecular pharmaceutics. 16:4241-4251.
Abstract
In diabetes, abnormal angiogenesis due to hyperglycemia and endothelial dysfunction impairs wound healing and results in high risks of diabetic foot ulcers and mortality. Alternative therapeutic methods were attempted to prevent diabetic complications through the activation of endothelial nitric oxide synthase. In this study, direct application of nitric oxide using dinitrosyl iron complexes (DNICs) to promote angiogenesis and wound healing under physiological conditions and in diabetic mice is investigated. Based on in vitro and in vivo studies, DNIC [Fe2(μ-SCH2CH2OH)2(NO)4] (DNIC-1) with a sustainable NO-release reactivity (t1/2 = 27.4 ± 0.5 h at 25 °C and 16.8 ± 1.8 h at 37 °C) activates the NO-sGC-cGMP pathway and displays the best pro-angiogenesis activity overwhelming other NO donors and the vascular endothelial growth factor. Moreover, this pro-angiogenesis effect of DNIC-1 restores the impaired angiogenesis in the ischemic hind limb and accelerates the recovery rate of wound closure in diabetic mice. This study translates synthetic DNIC-1 into a novel therapeutic agent for the treatment of diabetes and highlights its sustainable •NO-release reactivity on the activation of angiogenesis and wound healing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping