PUBLICATION

Nesfatin-1-Like Peptide Suppresses Hypothalamo-Pituitary-Gonadal mRNAs, Gonadal Steroidogenesis and Oocyte Maturation in Fish

Authors
Rajeswari, J.J., Hatef, A., Unniappan, S.
ID
ZDB-PUB-200617-16
Date
2020
Source
Biology of reproduction   103(4): 802-816 (Journal)
Registered Authors
Unniappan, Suraj
Keywords
HPG axis, Nesfatin-1-like peptide, Nucleobindins, goldfish, nesfatin-1, oocyte maturation, sex steroids
MeSH Terms
  • Animals
  • Aromatase/genetics
  • Aromatase/metabolism
  • Brain/enzymology
  • Down-Regulation/drug effects
  • Estradiol/blood
  • Female
  • Gene Expression Regulation/drug effects
  • Goldfish*
  • Gonadal Steroid Hormones/genetics
  • Gonadal Steroid Hormones/metabolism*
  • Gonadotropin-Releasing Hormone/genetics
  • Gonadotropin-Releasing Hormone/metabolism
  • Gonads
  • Hypothalamo-Hypophyseal System
  • Kisspeptins/genetics
  • Kisspeptins/metabolism
  • Male
  • Neuropeptides/genetics
  • Neuropeptides/metabolism
  • Nucleobindins/pharmacology*
  • Oocytes/physiology*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Receptors, Neuropeptide/genetics
  • Receptors, Neuropeptide/metabolism
  • Sex Factors
  • Testosterone/blood
  • Zebrafish
  • Zebrafish Proteins/pharmacology*
PubMed
32542346 Full text @ Biol. Reprod.
Abstract
Nucleobindin (Nucb)-1 and Nucb2 are DNA and Ca2+ binding proteins with multiple functions in vertebrates. Prohormone convertase-mediated processing of Nucb2 results in the production of biologically active nesfatin-1. Nesfatin-1 is involved in the regulation of reproduction in many vertebrates, including fish. Our lab originally reported a nesfatin-1-like peptide (Nlp) encoded in Nucb1 that exhibits nesfatin-1-like metabolic effects. We hypothesized that Nlp has a suppressive role in the reproductive physiology of fish. In this research, whether Nlp regulates reproductive hormones and oocyte maturation in fish was determined. Single intraperitoneal (I.P) injection of goldfish Nlp (50 ng/g body weight) suppressed salmon and chicken gonadotropin releasing hormone (sgnrh and cgnrh2), gonadotropin inhibiting hormone (gnih) and its receptor (gnihr), kisspeptin and brain aromatase mRNA expression in the hypothalamus of both male and female goldfish. In the pituitary, Nlp decreased mRNAs encoding lhb, fshb and kisspeptin and its receptor, while a significant increase in gnih and gnihr was observed. In the gonads, lh (only in male fish) and fsh receptor mRNAs were also significantly downregulated in Nlp injected fish. Sex-specific modulation of gnih, gnihr and kisspeptin system in the gonads was also observed. Nlp decreased sex steroidogenic enzyme encoding mRNAs, and circulating levels of testosterone and estradiol. In addition, incubation of zebrafish ovarian follicles with Nlp resulted in a reduction in oocyte maturation. These results provide evidence for a robust role for Nlp in regulating reproductive hormones in goldfish, and oocyte maturation in zebrafish, and these effects resemble that of nesfatin-1.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping