PUBLICATION
High-resolution mapping of injury-site dependent functional recovery in a single axon in zebrafish
- Authors
- Hecker, A., Anger, P., Braaker, P.N., Schulze, W., Schuster, S.
- ID
- ZDB-PUB-200615-4
- Date
- 2020
- Source
- Communications biology 3: 307 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axons/physiology*
- Larva/physiology*
- Larva/radiation effects
- Lasers/adverse effects
- Microscopy, Fluorescence, Multiphoton/methods*
- Nerve Regeneration*
- Recovery of Function*
- Spinal Cord Injuries/physiopathology*
- Zebrafish
- PubMed
- 32533058 Full text @ Commun Biol
Citation
Hecker, A., Anger, P., Braaker, P.N., Schulze, W., Schuster, S. (2020) High-resolution mapping of injury-site dependent functional recovery in a single axon in zebrafish. Communications biology. 3:307.
Abstract
In non-mammalian vertebrates, some neurons can regenerate after spinal cord injury. One of these, the giant Mauthner (M-) neuron shows a uniquely direct link to a robust survival-critical escape behavior but appears to regenerate poorly. Here we use two-photon microscopy in parallel with behavioral assays in zebrafish to show that the M-axon can regenerate very rapidly and that the recovery of functionality lags by just days. However, we also find that the site of the injury is critical: While regeneration is poor both close and far from the soma, rapid regeneration and recovery of function occurs for injuries between 10% and 50% of total axon length. Our findings show that rapid regeneration and the recovery of function can be studied at remarkable temporal resolution after targeted injury of one single M-axon and that the decision between poor and rapid regeneration can be studied in this one axon.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping