|ZFIN ID: ZDB-PUB-200604-2|
A versatile, multi-laser twin-microscope system for light-sheet imaging
Keomanee-Dizon, K., Fraser, S.E., Truong, T.V.
|Source:||The Review of scientific instruments 91: 053703 (Journal)|
|Registered Authors:||Fraser, Scott E.|
|PubMed:||32486724 Full text @ Rev. Sci. Instrum.|
Keomanee-Dizon, K., Fraser, S.E., Truong, T.V. (2020) A versatile, multi-laser twin-microscope system for light-sheet imaging. The Review of scientific instruments. 91:053703.
ABSTRACTLight-sheet microscopy offers faster imaging and reduced phototoxicity in comparison to conventional point-scanning microscopy, making it a preferred technique for imaging biological dynamics for durations of hours or days. Such extended imaging sessions pose a challenge, as it reduces the number of specimens that can be imaged in a given day. Here, we present a versatile light-sheet imaging instrument that combines two independently controlled microscope-twins, built so that they can share an ultrafast near-infrared laser and a bank of continuous-wave visible lasers, increasing the throughput and decreasing the cost. To permit a wide variety of specimens to be imaged, each microscope-twin provides flexible imaging parameters, including (i) operation in one-photon and/or two-photon excitation modes, (ii) delivery of one to three light-sheets via a trio of orthogonal excitation arms, (iii) sub-micron to micron imaging resolution, (iv) multicolor compatibility, and (v) upright (with provision for inverted) detection geometry. We offer a detailed description of the twin-microscope design to aid instrument builders who wish to construct and use similar systems. We demonstrate the instrument's versatility for biological investigation by performing fast imaging of the beating heart in an intact zebrafish embryo, deep imaging of thick patient-derived tumor organoids, and gentle whole-brain imaging of neural activity in behaving larval zebrafish.