PUBLICATION
Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H+-ATPase Rich Ionocytes
- Authors
- Tong, S.K., Lee, H.L., Lee, Y.C., Wu, L.C., Tsou, Y.L., Lu, S.W., Shih, S.W., Hwang, P.P., Chou, M.Y.
- ID
- ZDB-PUB-200604-1
- Date
- 2020
- Source
- International Journal of Molecular Sciences 21(11): (Journal)
- Registered Authors
- Chou, Ming-Yi, Hwang, Pung Pung
- Keywords
- and ion regulation, ionocyte, vasopressin
- MeSH Terms
-
- Acid-Base Equilibrium*
- Animals
- Arginine Vasopressin/pharmacology*
- Calcitonin Gene-Related Peptide/metabolism*
- Calcium/chemistry
- Chlorides/chemistry
- DNA, Complementary/metabolism
- Down-Regulation
- Electrodes
- Homeostasis
- In Situ Hybridization
- Ion Transport
- Ions/chemistry*
- Oligonucleotides, Antisense/pharmacology
- RNA, Messenger/metabolism
- Skin/metabolism
- Sodium/chemistry
- Sodium Chloride Symporters/metabolism*
- Vasopressins/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 32486459 Full text @ Int. J. Mol. Sci.
Citation
Tong, S.K., Lee, H.L., Lee, Y.C., Wu, L.C., Tsou, Y.L., Lu, S.W., Shih, S.W., Hwang, P.P., Chou, M.Y. (2020) Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H+-ATPase Rich Ionocytes. International Journal of Molecular Sciences. 21(11).
Abstract
Arginine vasopressin (Avp) is a conserved pleiotropic hormone that is known to regulate both water reabsorption and ion balance; however, many of the mechanisms underlying its effects remain unclear. Here, we used zebrafish embryos to investigate how Avp modulates ion and acid-base homeostasis. After incubating embryos in double-deionized water for 24 h, avp mRNA expression levels were significantly upregulated. Knockdown of Avp protein expression by an antisense morpholino oligonucleotide (MO) reduced the expression of ionocyte-related genes and downregulated whole-body Cl- content and H+ secretion, while Na+ and Ca2+ levels were not affected. Incubation of Avp antagonist SR49059 also downregulated the mRNA expression of sodium chloride cotransporter 2b (ncc2b), which is a transporter responsible for Cl- uptake. Correspondingly, avp morphants showed lower NCC and H+-ATPase rich (HR) cell numbers, but Na+/K+-ATPase rich (NaR) cell numbers remained unchanged. avp MO also downregulated the numbers of foxi3a- and p63-expressing cells. Finally, the mRNA expression levels of calcitonin gene-related peptide (cgrp) and its receptor, calcitonin receptor-like 1 (crlr1), were downregulated in avp morphants, suggesting that Avp might affect Cgrp and Crlr1 for modulating Cl- balance. Together, our results reveal a molecular/cellular pathway through which Avp regulates ion and acid-base balance, providing new insights into its function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping