ZFIN ID: ZDB-PUB-200604-1
Arginine Vasopressin Modulates Ion and Acid/Base Balance by Regulating Cell Numbers of Sodium Chloride Cotransporter and H+-ATPase Rich Ionocytes
Tong, S.K., Lee, H.L., Lee, Y.C., Wu, L.C., Tsou, Y.L., Lu, S.W., Shih, S.W., Hwang, P.P., Chou, M.Y.
Date: 2020
Source: International Journal of Molecular Sciences   21(11): (Journal)
Registered Authors: Chou, Ming-Yi, Hwang, Pung Pung
Keywords: and ion regulation, ionocyte, vasopressin
MeSH Terms:
  • Acid-Base Equilibrium*
  • Animals
  • Arginine Vasopressin/pharmacology*
  • Calcitonin Gene-Related Peptide/metabolism*
  • Calcium/chemistry
  • Chlorides/chemistry
  • DNA, Complementary/metabolism
  • Down-Regulation
  • Electrodes
  • Homeostasis
  • In Situ Hybridization
  • Ion Transport
  • Ions/chemistry*
  • Oligonucleotides, Antisense/pharmacology
  • RNA, Messenger/metabolism
  • Skin/metabolism
  • Sodium/chemistry
  • Sodium Chloride Symporters/metabolism*
  • Vasopressins/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
PubMed: 32486459 Full text @ Int. J. Mol. Sci.
ABSTRACT
Arginine vasopressin (Avp) is a conserved pleiotropic hormone that is known to regulate both water reabsorption and ion balance; however, many of the mechanisms underlying its effects remain unclear. Here, we used zebrafish embryos to investigate how Avp modulates ion and acid-base homeostasis. After incubating embryos in double-deionized water for 24 h, avp mRNA expression levels were significantly upregulated. Knockdown of Avp protein expression by an antisense morpholino oligonucleotide (MO) reduced the expression of ionocyte-related genes and downregulated whole-body Cl- content and H+ secretion, while Na+ and Ca2+ levels were not affected. Incubation of Avp antagonist SR49059 also downregulated the mRNA expression of sodium chloride cotransporter 2b (ncc2b), which is a transporter responsible for Cl- uptake. Correspondingly, avp morphants showed lower NCC and H+-ATPase rich (HR) cell numbers, but Na+/K+-ATPase rich (NaR) cell numbers remained unchanged. avp MO also downregulated the numbers of foxi3a- and p63-expressing cells. Finally, the mRNA expression levels of calcitonin gene-related peptide (cgrp) and its receptor, calcitonin receptor-like 1 (crlr1), were downregulated in avp morphants, suggesting that Avp might affect Cgrp and Crlr1 for modulating Cl- balance. Together, our results reveal a molecular/cellular pathway through which Avp regulates ion and acid-base balance, providing new insights into its function.
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