ZFIN ID: ZDB-PUB-200603-1
Characterization and toxicology evaluation of low molecular weight chitosan on zebrafish
Chou, C.M., Mi, F.L., Horng, J.L., Lin, L.Y., Tsai, M.L., Liu, C.L., Lu, K.Y., Chu, C.Y., Chen, Y.T., Lee, Y.A., Cheng, C.H.
Date: 2020
Source: Carbohydrate Polymers   240: 116164 (Journal)
Registered Authors: Cheng, Chia-Hsiung, Horng, Jiun-Lin
Keywords: Low-molecular-weight chitosan, Nanoparticle, Toxicity, Zebrafish
MeSH Terms:
  • Animals
  • Biocompatible Materials/toxicity*
  • Cell Line
  • Cell Membrane/drug effects
  • Chitosan/analogs & derivatives
  • Chitosan/toxicity*
  • Epithelial Cells/cytology
  • Epithelial Cells/drug effects
  • Larva/drug effects*
  • Liver/drug effects*
  • Molecular Weight
  • Toxicity Tests
  • Zebrafish/metabolism*
PubMed: 32475540 Full text @ Carbohydr. Polym.
Chitosan is suggested as no or low toxicity and biocompatible biomaterial. Digestion of chitosan to reduce molecular weight and formulate nanoparticle was generally used to improve efficiency for DNA or protein delivery. However, the toxicity of low-molecular-weight chitosan (LMWCS) towards freshwater fishes has not been well evaluated. Here, we reported the toxic mechanism of LMWCS using zebrafish (Danio rerio) liver (ZFL) cell line, zebrafish larvae, and adult fish. LMWCS rapidly induced cytotoxicity of ZFL cells and death of zebrafish. Cell membrane damaged by LMWCS reduced cell viability. Damaged membrane of epithelial cell in zebrafish larvae induced breakage of the yolk. Adult fish exhibited hypoxia before death due to multiple damages induced by LMWCS. Although the toxicity of LMWCS was revealed in zebrafish model, the toxicity was only present in pH < 7 and easy be neutralized by other negative ions. Collectively, these data improved a new understanding of LMWCS properties.