PUBLICATION
Environmentally relevant concentrations of geosmin affect the development, oxidative stress, apoptosis and endocrine disruption of embryo-larval zebrafish
- Authors
- Zhou, W., Wang, J., Zhang, J., Peng, C., Li, G., Li, D.
- ID
- ZDB-PUB-200601-12
- Date
- 2020
- Source
- The Science of the total environment 735: 139373 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Geosmin, Growth, Oxidative stress, Zebrafish embryo
- MeSH Terms
-
- Animals
- Apoptosis
- Ecosystem
- Embryo, Nonmammalian
- Larva
- Naphthols
- Oxidative Stress
- Water Pollutants, Chemical*
- Zebrafish*
- PubMed
- 32473435 Full text @ Sci. Total Environ.
Citation
Zhou, W., Wang, J., Zhang, J., Peng, C., Li, G., Li, D. (2020) Environmentally relevant concentrations of geosmin affect the development, oxidative stress, apoptosis and endocrine disruption of embryo-larval zebrafish. The Science of the total environment. 735:139373.
Abstract
Geosmin (trans-1, 10-dimethyl-trans-9-decalol), a volatile organic compound, has been widely detected in aquatic ecosystems. However, the ecological effects of geosmin are not clear. Here, using zebrafish (Danio rerio) embryo as a model, we investigated biological activity effects of environmentally relevant concentrations (50, 500, 5000 ng/L) of geosmin on the developing zebrafish starting from 2 h post-fertilization (hpf) to 96 hpf. Results showed geosmin had no effect on hatchability, malformations and mortality. However, we observed that geosmin exposure significantly increased zebrafish body length in a concentration dependent manner. This effect was possibly due to up-regulation of expression of genes along the growth hormone/insulin-like growth factor (GH/IGF) axis and hypothalamic-pituitary-thyroid (HPT) axis. In addition, superoxide dismutase (SOD) activities and catalase (CAT) activities significantly increased at 96 hpf when the embryos were exposed to 500 and 5000 ng/L of geosmin. The malondialdehyde (MDA) contents and glutathione S-transferase (GST) activities decreased significantly after the exposure to 5000 ng/L geosmin. Simultaneously, exposure to geosmin resulted in significant increase in cell apoptosis, mainly in the heart area. The mRNA levels of the genes related to oxidative stress and apoptosis were also altered significantly after geosmin exposure. These findings indicated that geosmin can simultaneously induce multiple responses during zebrafish embryonic development, including oxidative stress, apoptosis, and endocrine disruption.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping