PUBLICATION
Acute and long-term metabolic consequences of early developmental Bisphenol A exposure in zebrafish (Danio rerio)
- Authors
- Martínez, R., Tu, W., Eng, T., Allaire-Leung, M., Piña, B., Navarro-Martín, L., Mennigen, J.A.
- ID
- ZDB-PUB-200526-17
- Date
- 2020
- Source
- Chemosphere 256: 127080 (Journal)
- Registered Authors
- Piña, Benjamin
- Keywords
- BPA, Estrogenic chemicals, Metabolic disruption, Metabolism, Plasticizer, microRNA
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/toxicity*
- Larva/drug effects
- Metabolism/drug effects*
- Phenols/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- PubMed
- 32450349 Full text @ Chemosphere
Citation
Martínez, R., Tu, W., Eng, T., Allaire-Leung, M., Piña, B., Navarro-Martín, L., Mennigen, J.A. (2020) Acute and long-term metabolic consequences of early developmental Bisphenol A exposure in zebrafish (Danio rerio). Chemosphere. 256:127080.
Abstract
Bisphenol A (BPA) is an estrogenic contaminant linked to metabolic disruption. Developmental BPA exposure is of particular concern, as organizational effects may irreversibly disrupt metabolism at later life-stages. While BPA exposures in adult fish elicit metabolic perturbations similar to effects described in rodents, the metabolic effects of developmental BPA exposure in juvenile fish remain largely unknown. Following embryonic zebrafish exposure to BPA (0.1, 1 and 4 mg/L) and EE2 (10 ng/L) from 2 to 5 dpf, we assessed the metabolic phenotype in larvae (4-6 dpf) and juveniles (43-49 dpf) which had been divided into regular-fed and overfed groups at 29 dpf. Developmental BPA exposure in larvae dose-dependently reduced food-intake and locomotion and increased energy expenditure. Juveniles (29 dpf) exhibited a transient increase in body weight after developmental BPA exposure and persistent diet-dependent locomotion changes (43-49 dpf). At the molecular level, glucose and lipid metabolism-related transcript abundance clearly separated BPA exposed fish from controls and EE2 exposed fish at the larval stage, in juveniles on a regular diet and, to a lesser extent, in overfed juveniles. In general, the metabolic endpoints affected by BPA exposure were not mimicked by EE2 treatment. We conclude that developmental BPA exposure elicits acute metabolic effects in zebrafish larvae and fewer transient and persistent effects in juveniles and that these metabolic effects are largely independent of BPA's estrogenicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping