PUBLICATION
Effect of intracerebroventricular administration of two molecular forms of sulfated CCK octapeptide on anxiety-like behavior in the zebrafish danio rerio
- Authors
- Matsuda, K., Yoshida, D., Sachuriga, ., Watanabe, K., Yokobori, E., Konno, N., Nakamachi, T.
- ID
- ZDB-PUB-200524-24
- Date
- 2020
- Source
- Peptides 130: 170330 (Journal)
- Registered Authors
- Matsuda, Kouhei, Yoshida, Tomoyuki
- Keywords
- Anxiety-Like behavior, Brain, CCK-like immunoreactivity, CCKA-8s, CCKB-8s, Cholecystokinin, ICV administration, Preference test, zebra fish
- MeSH Terms
-
- Animals
- Anxiety/chemically induced*
- Behavior, Animal/drug effects*
- Benzodiazepines/pharmacology
- Brain/metabolism*
- Carbolines/pharmacology
- Injections, Intraventricular
- Locomotion/drug effects
- Proglumide/pharmacology
- Sincalide/administration & dosage
- Sincalide/analogs & derivatives*
- Sincalide/metabolism
- Sincalide/pharmacology
- Zebrafish*
- Zebrafish Proteins/metabolism
- PubMed
- 32445877 Full text @ Peptides
Citation
Matsuda, K., Yoshida, D., Sachuriga, ., Watanabe, K., Yokobori, E., Konno, N., Nakamachi, T. (2020) Effect of intracerebroventricular administration of two molecular forms of sulfated CCK octapeptide on anxiety-like behavior in the zebrafish danio rerio. Peptides. 130:170330.
Abstract
Cholecystokinin octapeptide with sulfate (CCK-8 s) regulates feeding behavior and psychomotor activity. In rodents and goldfish, intracerebroventricular (ICV) injection of CCK-8 s decreases food intake and also induces anxiety-like behavior. The zebrafish has several merits for investigating the psychophysiological roles of neuropeptides. However, little is known about the brain localization of CCK and the behavioral action of CCK-8 s in this species. Here we investigated the brain localization of CCK-like immunoreactivity and found that it was distributed throughout the brain. As CCK-like immunoreactivity was particularly evident in the ventral habenular nucleus, the interpeduncular nucleus and superior raphe, we subsequently examined the effect of zebrafish (zf) CCK-8 s on psychomotor control. Since the zebrafish possesses two molecular forms of zfCCK-8 s (zfCCKA-8 s and zfCCKB-8 s), two synthetic peptides were administered intracerebroventricularly at 1, 5 and 10 pmol g-1 body weight (BW). As the zebrafish shows a greater preference for the lower area of a tank than for to the upper area, we used this preference for assessment of anxiety-like behavior. ICV administration of zfCCKA-8 s or zfCCKB-8 s at 10 pmol g-1 BW significantly shortened the time spent in the upper area. The actions of these peptides mimicked that of the central-type benzodiazepine receptor inverse agonist FG-7142 (an anxiogenic agent) at 10 pmol g-1 BW. The anxiogenic-like action of the two peptides was attenuated by treatment with the CCK receptor antagonist proglumide at 200 pmol g-1 BW. These results indicate that zfCCKA-8 s and zfCCKB-8 s potently induce anxiety-like behavior via the CCK receptor-signaling pathway in the zebrafish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping