PUBLICATION
Development of vascular regulation in the zebrafish embryo
- Authors
- Bahrami, N., Childs, S.J.
- ID
- ZDB-PUB-200520-6
- Date
- 2020
- Source
- Development (Cambridge, England) 147(10): (Journal)
- Registered Authors
- Childs, Sarah J.
- Keywords
- Astrocyte, Blood vessel, Contraction, Pericyte, Relaxation, Vascular smooth muscle cell (vSMC), Vasomotor activity, Zebrafish
- MeSH Terms
-
- Adrenergic alpha-1 Receptor Agonists/pharmacology
- Animals
- Animals, Genetically Modified
- Brain/blood supply
- Brain/diagnostic imaging
- Brain/embryology
- Endothelial Cells/physiology
- Endothelium, Vascular/embryology
- Gene Silencing
- Metronidazole/pharmacology
- Muscle Contraction/drug effects
- Muscle, Smooth, Vascular/embryology*
- Myocytes, Smooth Muscle/physiology*
- Nitric Oxide Donors/pharmacology
- Pericytes/physiology*
- Vasodilation/drug effects
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 32423977 Full text @ Development
Citation
Bahrami, N., Childs, S.J. (2020) Development of vascular regulation in the zebrafish embryo. Development (Cambridge, England). 147(10):.
Abstract
The thin endothelial wall of a newly formed vessel is under enormous stress at the onset of blood flow, rapidly acquiring support from mural cells (pericytes and vascular smooth muscle cells; vSMCs) during development. Mural cells then develop vasoactivity (contraction and relaxation) but we have little information as to when this first develops or the extent to which pericytes and vSMCs contribute. For the first time, we determine the dynamic developmental acquisition of vasoactivity in vivo in the cerebral vasculature of zebrafish. We show that pericyte-covered vessels constrict in response to α1-adrenergic receptor agonists and dilate in response to nitric oxide donors at 4 days postfertilization (dpf) but have heterogeneous responses later, at 6 dpf. In contrast, vSMC-covered vessels constrict at 6 dpf, and dilate at both stages. Using genetic ablation, we demonstrate that vascular constriction and dilation is an active response. Our data suggest that both pericyte- and vSMC-covered vessels regulate their diameter in early development, and that their relative contributions change over developmental time.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping