PUBLICATION
Deletion of c16orf45 in zebrafish results in a low fertilization rate and increased thigmotaxis
- Authors
- Li, T., Li, F., Lin, J., Zhang, Y., Zhang, Q., Sun, Y., Chen, X., Xu, M., Wang, X., Li, Q.
- ID
- ZDB-PUB-200519-4
- Date
- 2020
- Source
- Developmental psychobiology 62(8): 1003-1010 (Journal)
- Registered Authors
- Li, Qiang
- Keywords
- c16orf45, CRISPR/Cas9, fertilization rate, thigmotaxis, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Behavior, Animal/physiology*
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Disease Models, Animal
- Embryo, Nonmammalian
- Exoribonucleases/physiology*
- Female
- Fertility/genetics*
- Fertilization/genetics*
- Gene Deletion
- Male
- Neurodevelopmental Disorders/genetics*
- Zebrafish
- Zebrafish Proteins/physiology*
- PubMed
- 32421859 Full text @ Dev Psychobiol
Citation
Li, T., Li, F., Lin, J., Zhang, Y., Zhang, Q., Sun, Y., Chen, X., Xu, M., Wang, X., Li, Q. (2020) Deletion of c16orf45 in zebrafish results in a low fertilization rate and increased thigmotaxis. Developmental psychobiology. 62(8):1003-1010.
Abstract
c16orf45 is located at 16p13.11, an important locus related to neurodevelopmental diseases. Clinical studies have demonstrated that c16orf45 is associated with various neurodevelopmental diseases. To further elucidate the effect of c16orf45 on neural development, we constructed a zebrafish model with a stably inherited c16orf45 deletion via CRISPR/Cas9 technology. We found that deletion of c16orf45 significantly reduced the zebrafish fertilization rate, and both females and males showed reduced fertility. Meanwhile, the homozygous c16orf45 knockout zebrafish showed a developmental delay at 24 hr postfertilization (hpf). However, morphological changes were not apparent after 2 days postfertilization (dpf). Notably, the results of behavioral experiments revealed increased thigmotaxis in c16orf45-/- zebrafish at 2 months. In conclusion, these findings demonstrate that c16orf45 plays an important role in nervous system and reproductive system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping