PUBLICATION
Identification of β tubulin IVb as a pattern recognition receptor with opsonic activity
- Authors
- Duan, H., Yang, S., Ni, S., Ma, Z., Yuan, J., Zhang, S.
- ID
- ZDB-PUB-200511-7
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 235: 108781 (Journal)
- Registered Authors
- Keywords
- LPS/LTA, Opsonin, Pattern recognition receptor, Tubulin, Zebrafish
- MeSH Terms
-
- Agglutination
- Animals
- Bacteria/metabolism*
- Gene Expression Regulation
- Lipopolysaccharides/metabolism
- Opsonin Proteins/genetics
- Opsonin Proteins/metabolism*
- Phagocytosis*
- Receptors, Pattern Recognition/genetics
- Receptors, Pattern Recognition/metabolism*
- Teichoic Acids/metabolism
- Tubulin/genetics
- Tubulin/metabolism*
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32387479 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Duan, H., Yang, S., Ni, S., Ma, Z., Yuan, J., Zhang, S. (2020) Identification of β tubulin IVb as a pattern recognition receptor with opsonic activity. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 235:108781.
Abstract
Previous studies have shown that tubulins play important role in immune responses of both plants and animals, but no experiments have been performed to study the mode of action of tubulins in immune defense. In addition, there is little convincing experimental evidence of functional commitment for specific tubulin isotypes in animals. In the present, we showed that expression of β-tubulin IVb gene was affected by both LPS and LTA, hinting its involvement in anti-infectious response. We also showed that recombinant zebrafish β-tubulin IVb not only interacted with LPS and LTA as well as Gram-negative and -positive bacteria but also agglutinated both Gram-negative and -positive bacteria in a Ca2+-dependent fashion. Interestingly, recombinant β-tubulin IVb could enhance the phagocytosis of bacteria by macrophages. Moreover, we demonstrated that β-tubulin IVb was present extracellularly in the serum of zebrafish and mouse. Collectively, these suggest that β-tubulin IVb may be physiologically involved in the systematic immunity of host via acting as a pattern recognition receptor and an opsonin. This also provides a new angle to understand the roles of β-tubulin IVb.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping