PUBLICATION
Influence of 3-nitropropionic acid on physiological and behavioral responses in zebrafish larvae and adults
- Authors
- Wiprich, M.T., Zanandrea, R., Altenhofen, S., Bonan, C.D.
- ID
- ZDB-PUB-200501-7
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 234: 108772 (Journal)
- Registered Authors
- Bonan, Carla Denise
- Keywords
- 3-Nitropropionic acid, Huntington's disease, Locomotion, Memory, Zebrafish
- MeSH Terms
-
- Aggression/drug effects
- Animals
- Antihypertensive Agents/pharmacology
- Behavior, Animal/drug effects*
- Disease Models, Animal
- Heart Rate/drug effects
- Larva/drug effects
- Locomotion/drug effects
- Motor Activity/drug effects
- Nitro Compounds/pharmacology*
- Propionates/pharmacology*
- Zebrafish/embryology
- Zebrafish/physiology
- PubMed
- 32353558 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Wiprich, M.T., Zanandrea, R., Altenhofen, S., Bonan, C.D. (2020) Influence of 3-nitropropionic acid on physiological and behavioral responses in zebrafish larvae and adults. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 234:108772.
Abstract
Long-term treatment with 3-nitropropionic acid (3-NPA), a toxin derived from plants and fungi, may reproduce symptoms and biochemical characteristics of Huntington's disease (HD). Our study evaluated the effects of 3-NPA on the physiological and behavioral responses in zebrafish larvae and adults. Larvae exposed to 0.1, 0.2, or 0.5 mM 3-NPA exhibited an increase in heart rate at 2- and 5-days post-fertilization (dpf). There was a decrease in the ocular distance at 5 dpf with 0.05 mM 3-NPA treatment. However, 3-NPA did not alter larval locomotor parameters. Adult zebrafish received 3-NPA intraperitoneal injections (a total of seven injections at doses 10, 20, or 60 mg/kg every 96 h) and showed a decrease in weight loss, locomotion and aggressive behavior. No changes were observed in anxiety-like behavior and social interaction between 3-NPA-exposed animals and control groups. However, 3-NPA-treated animals (at 60 mg/kg) demonstrated impaired long-term aversive memory. Overall, 3-NPA exposure induced morphological and heart rate alterations in zebrafish larvae. Additionally, our study showed behavioral changes in zebrafish that were submitted to long-term 3-NPA treatment, which could be related to HD symptoms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping