PUBLICATION
Orbencarb induces lethality and organ malformation in zebrafish embryos during development
- Authors
- Lee, J.Y., Park, S., Lim, W., Song, G.
- ID
- ZDB-PUB-200427-2
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 233: 108771 (Journal)
- Registered Authors
- Keywords
- Development, Embryotoxicity, Orbencarb, Vasculogenesis, Zebrafish
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Embryo, Nonmammalian/drug effects
- Embryonic Development/drug effects*
- Intestines/drug effects
- Neovascularization, Physiologic/drug effects
- Pesticides/toxicity*
- Reactive Oxygen Species/metabolism
- Thiocarbamates/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- PubMed
- 32335232 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Lee, J.Y., Park, S., Lim, W., Song, G. (2020) Orbencarb induces lethality and organ malformation in zebrafish embryos during development. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 233:108771.
Abstract
Thiocarbamates are one of the components of pesticides that target weeds by inhibiting adenosine triphosphate (ATP) synthesis. Orbencarb, one of the isomeric thiocarbamates applied to wheat, maize, and soybean, has been found to have toxic effects on mammals and marine ecosystems. Although the toxicity ranges of orbencarb in different organisms are known, specific studies on the environmental contamination and harmful effects of orbencarb on non-target organisms are scarce. In this study, we observed that orbencarb induced embryotoxicity during zebrafish development as well as apoptosis and reactive oxygen species (ROS) production in the intestine. It was further observed that orbencarb decreased the viability of the embryos and simultaneously affected the heart rate and vessel formation. Orbencarb decreased the mRNA levels of ccnd1, ccne1, cdk2, and cdk6 and induced abnormal development of the eyes, brain, yolk sac, and spinal cord in zebrafish embryos. Orbencarb also hampered vasculogenesis in the zebrafish embryos by inhibiting the mRNA expression of flt1, flt4, kdr, and vegfc. Collectively, these results suggested that orbencarb is embryotoxic and disrupts the normal growth of zebrafish embryos by inducing the generation of ROS and hampering vasculogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping