PUBLICATION
The Impact on Antioxidant Enzyme Activity and Related Gene Expression Following Adult Zebrafish (Danio rerio) Exposure to Dimethyl Phthalate
- Authors
- Cong, B., Liu, C., Wang, L., Chai, Y.
- ID
- ZDB-PUB-200426-6
- Date
- 2020
- Source
- Animals : an open access journal from MDPI 10(4): (Journal)
- Registered Authors
- Keywords
- DMP, antioxidant enzyme activity, gene expression, zebrafish
- MeSH Terms
- none
- PubMed
- 32325949 Full text @ Animals (Basel)
Citation
Cong, B., Liu, C., Wang, L., Chai, Y. (2020) The Impact on Antioxidant Enzyme Activity and Related Gene Expression Following Adult Zebrafish (Danio rerio) Exposure to Dimethyl Phthalate. Animals : an open access journal from MDPI. 10(4):.
Abstract
Dimethyl phthalate (DMP) is a widespread environmental contaminant that poses potential toxicity risks for animals and humans. However, the toxicological effects of DMP on fish have not been adequately examined. In this study, the acute toxicity, oxidative damage, antioxidant enzyme activities, and relative gene expression patterns were investigated in the liver of adult zebrafish (Danio rerio) exposed to DMP. We found that the lethal concentration (LC50) of DMP for zebrafish after 96 h of exposure was 45.8 mg/L. The zebrafish that were exposed to low, medium and high concentrations of DMP (0.5, 4.6, and 22.9 mg/L, respectively) for 96 h had an increased malondialdehyde (MDA) content and a lower antioxidant capacity compared with the control solvent group. The total superoxide dismutase (SOD) activity was significantly higher than 0 h after initial exposure for 24 h at low concentrations, and then decreased at high concentrations after exposure for 96 h. The catalase (CAT) and glutathione S-transferase (GST) activities were significantly reduced after 96 h of exposure to high concentrations of DMP, with the up- or down-regulation of the related transcriptional expression. These findings indicated that DMP could cause physiological effects in zebrafish by disturbing the expression levels of antioxidant enzymes. These results might contribute to the identification of biomarkers to monitor phthalate pollution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping