PUBLICATION
Zebrafish NF-κB/p65 Is Required for Antiviral Responses
- Authors
- Ouyang, G., Liao, Q., Zhang, D., Rong, F., Cai, X., Fan, S., Zhu, J., Wang, J., Liu, X., Liu, X., Xiao, W.
- ID
- ZDB-PUB-200424-2
- Date
- 2020
- Source
- Journal of immunology (Baltimore, Md. : 1950) 204(11): 3019-3029 (Journal)
- Registered Authors
- Ouyang, Gang, Rong, Fangjing, Wang, Jing, Xiao, Wuhan
- Keywords
- none
- MeSH Terms
-
- Zebrafish/immunology*
- Zebrafish/virology
- eIF-2 Kinase/genetics
- eIF-2 Kinase/metabolism*
- Animals
- Immunity, Innate
- Rhabdoviridae/physiology*
- Signal Transduction
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Cells, Cultured
- Gene Knockdown Techniques
- NF-kappa B/metabolism*
- Mice
- Inflammation/genetics
- Rhabdoviridae Infections/immunology*
- Interferons/genetics
- Fish Diseases/genetics
- Fish Diseases/metabolism*
- Animals, Genetically Modified
- Adaptive Immunity
- PubMed
- 32321758 Full text @ J. Immunol.
Citation
Ouyang, G., Liao, Q., Zhang, D., Rong, F., Cai, X., Fan, S., Zhu, J., Wang, J., Liu, X., Liu, X., Xiao, W. (2020) Zebrafish NF-κB/p65 Is Required for Antiviral Responses. Journal of immunology (Baltimore, Md. : 1950). 204(11):3019-3029.
Abstract
Transcriptional programs regulated by the NF-κB family are essential for the inflammatory response as well as for innate and adaptive immunity. NF-κB activation occurs via two major signaling pathways: the canonical and the noncanonical. The canonical NF-κB pathway responds to diverse immune stimulations and leads to rapid but transient activation. As a member of the canonical NF-κB family, p65 is thought to be a key regulator of viral infection. Because of the embryonic lethality of p65-null mice, the physiological role of p65 in the antiviral immune response is still unclear. In this study, we generated p65-null zebrafish, which were viable and indistinguishable from their wildtype (WT) siblings under normal conditions. However, p65-null zebrafish were more sensitive to spring viremia of carp virus infection than their WT siblings. Further assays indicated that proinflammatory and antiviral genes, including IFN, were downregulated in p65-null zebrafish after spring viremia of carp virus infection compared with their WT siblings. Our results thus suggested that p65 is required for the antiviral response, activating not only proinflammatory genes but also antiviral genes (including IFN).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping