PUBLICATION
Fenobucarb-induced developmental neurotoxicity and mechanisms in zebrafish
- Authors
- Zhu, X.Y., Wu, Y.Y., Xia, B., Dai, M.Z., Huang, Y.F., Yang, H., Li, C.Q., Li, P.
- ID
- ZDB-PUB-200422-29
- Date
- 2020
- Source
- Neurotoxicology 79: 11-19 (Journal)
- Registered Authors
- Keywords
- BPMC, Fenobucarb, Mechanisms, Neurotoxicity, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Apoptosis/drug effects
- Behavior, Animal/drug effects
- Carbamates/toxicity*
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/pathology
- Embryonic Development/drug effects*
- Gene Expression Regulation, Developmental
- Inflammation Mediators/metabolism
- Insecticides/toxicity*
- Locomotion/drug effects
- Nerve Degeneration
- Nervous System/drug effects*
- Nervous System/embryology
- Nervous System/metabolism
- Nervous System/pathology
- Oxidative Stress/drug effects
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 32247646 Full text @ Neurotoxicology
- CTD
- 32247646
Citation
Zhu, X.Y., Wu, Y.Y., Xia, B., Dai, M.Z., Huang, Y.F., Yang, H., Li, C.Q., Li, P. (2020) Fenobucarb-induced developmental neurotoxicity and mechanisms in zebrafish. Neurotoxicology. 79:11-19.
Abstract
Fenobucarb (2-sec-butylphenyl methylcarbamate, BPMC) is an extensively used carbamate insecticide. Its developmental neurotoxicity and the underlying mechanisms have not been well investigated. In this study, zebrafish embryos were exposed to various concentrations of BPMC from 6 hpf (hours post fertilization, hpf) to 120 hpf. BPMC induced developmental toxicity with reduced motility in larval zebrafish. The spinal cord neutrophil infiltration, increased ROS production, caspase 3 and 9 activation, central nerve and peripheral motor neuron damage, axon and myelin degeneration were observed in zebrafish treated with BPMC generally in a dose-dependent manner. The expression of eight marker genes for nervous system function or development, namely, a1-tubulin, shha, elavl3, gap43, syn2a, gfap, mbp and manf, was significantly downregulated following BPMC exposure. AChE activity reduction and ache gene expression suppression was also found significantly in BPMC-treated zebrafish. These results indicate that BPMC is highly toxic to zebrafish and that BPMC induces zebrafish developmental neurotoxicity through pathways involved in inflammation, oxidative stress, degeneration and apoptosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping