PUBLICATION
GPCR-independent activation of G proteins promotes apical cell constriction in vivo
- Authors
- Marivin, A., Morozova, V., Walawalkar, I., Leyme, A., Kretov, D.A., Cifuentes, D., Dominguez, I., Garcia-Marcos, M.
- ID
- ZDB-PUB-200422-183
- Date
- 2019
- Source
- The Journal of cell biology 218: 1743-1763 (Journal)
- Registered Authors
- Cifuentes, Daniel, Garcia-Marcos, Mikel
- Keywords
- none
- MeSH Terms
-
- Actomyosin/metabolism
- Animals
- Cells, Cultured
- Constriction
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/metabolism
- Guanine Nucleotide Exchange Factors/genetics
- Guanine Nucleotide Exchange Factors/metabolism
- Heterotrimeric GTP-Binding Proteins/genetics
- Heterotrimeric GTP-Binding Proteins/metabolism*
- Humans
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/metabolism*
- Microfilament Proteins/genetics
- Microfilament Proteins/metabolism*
- Morphogenesis*
- Neural Plate/cytology*
- Neural Plate/metabolism
- Neurulation
- Protein Interaction Domains and Motifs
- Receptors, G-Protein-Coupled/genetics
- Receptors, G-Protein-Coupled/metabolism*
- Signal Transduction
- Xenopus laevis/embryology
- Xenopus laevis/physiology
- Zebrafish/embryology
- Zebrafish/physiology
- PubMed
- 30948426 Full text @ J. Cell Biol.
Citation
Marivin, A., Morozova, V., Walawalkar, I., Leyme, A., Kretov, D.A., Cifuentes, D., Dominguez, I., Garcia-Marcos, M. (2019) GPCR-independent activation of G proteins promotes apical cell constriction in vivo. The Journal of cell biology. 218:1743-1763.
Abstract
Heterotrimeric G proteins are signaling switches that control organismal morphogenesis across metazoans. In invertebrates, specific GPCRs instruct G proteins to promote collective apical cell constriction in the context of epithelial tissue morphogenesis. In contrast, tissue-specific factors that instruct G proteins during analogous processes in vertebrates are largely unknown. Here, we show that DAPLE, a non-GPCR protein linked to human neurodevelopmental disorders, is expressed specifically in the neural plate of Xenopus laevis embryos to trigger a G protein signaling pathway that promotes apical cell constriction during neurulation. DAPLE localizes to apical cell-cell junctions in the neuroepithelium, where it activates G protein signaling to drive actomyosin-dependent apical constriction and subsequent bending of the neural plate. This function is mediated by a Gα-binding-and-activating (GBA) motif that was acquired by DAPLE in vertebrates during evolution. These findings reveal that regulation of tissue remodeling during vertebrate development can be driven by an unconventional mechanism of heterotrimeric G protein activation that operates in lieu of GPCRs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping