PUBLICATION
Long-term and in vivo assessment of Aβ protein induced brain atrophy in a zebrafish model by optical coherence tomography
- Authors
- Lin, Y., Chen, T., Mao, G., Qiu, T., Lan, Y., Xiang, X., Huang, J., Huang, J., Lu, T., Gan, S., Sun, X.D., Zhang, J.
- ID
- ZDB-PUB-200422-153
- Date
- 2020
- Source
- Journal of biophotonics 13(7): e202000067 (Journal)
- Registered Authors
- Zhang, Jian
- Keywords
- Aβ protein, atrophy, brain, optical coherence tomography, zebrafish
- MeSH Terms
-
- Amyloid beta-Peptides/toxicity
- Animals
- Atrophy
- Brain/diagnostic imaging
- Tomography, Optical Coherence*
- Zebrafish*
- PubMed
- 32306519 Full text @ J. Biophotonics
Citation
Lin, Y., Chen, T., Mao, G., Qiu, T., Lan, Y., Xiang, X., Huang, J., Huang, J., Lu, T., Gan, S., Sun, X.D., Zhang, J. (2020) Long-term and in vivo assessment of Aβ protein induced brain atrophy in a zebrafish model by optical coherence tomography. Journal of biophotonics. 13(7):e202000067.
Abstract
In this study, a neurotoxicity model of zebrafish induced by Aβ protein was developed and evaluated in vivo by optical coherence tomography (OCT). Aβ protein and PBS were separately injected into the head of two groups of adult zebrafish (n = 6 per group). Congo-red staining results confirmed that Aβ protein had penetrated into brain tissue. All zebrafish were imaged with OCT on the 0th, 5th, 10th, 15th, and 20th day post-injection. OCT images showed that PBS is not toxic to brain tissue. However, significant brain atrophy could be seen in the OCT images of zebrafish injected with Aβ-protein that was verified by histological consequences. In addition, zebrafish in the model group showed memory decline in behavioral tests. This study verified the feasibility of in vivo long-term assessment of Aβ protein induced brain atrophy in adult zebrafish by OCT that has great potential to be applied in the neurological diseases research. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping