PUBLICATION
Melatonin attenuates 17β-trenbolone induced insomnia-like phenotype and movement deficiency in zebrafish
- Authors
- Mi, P., Gao, Q., Feng, Z.Y., Zhang, J.W., Zhao, X., Chen, D.Y., Feng, X.Z.
- ID
- ZDB-PUB-200422-149
- Date
- 2020
- Source
- Chemosphere 253: 126762 (Journal)
- Registered Authors
- Keywords
- 17β-trenbolone, Locomotion, Melatonin, Sleep/wake behavior, Zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Cattle
- Circadian Rhythm/drug effects
- Circadian Rhythm/genetics
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/physiology
- Embryonic Development/drug effects
- Environmental Pollutants/toxicity*
- Gene Expression Regulation, Developmental/drug effects
- Melatonin/pharmacology*
- Motor Activity/drug effects*
- Motor Neurons/drug effects
- Orexins/genetics
- Phenotype
- Sleep Initiation and Maintenance Disorders/chemically induced
- Sleep Initiation and Maintenance Disorders/prevention & control*
- Trenbolone Acetate/toxicity*
- Zebrafish*
- PubMed
- 32302915 Full text @ Chemosphere
Citation
Mi, P., Gao, Q., Feng, Z.Y., Zhang, J.W., Zhao, X., Chen, D.Y., Feng, X.Z. (2020) Melatonin attenuates 17β-trenbolone induced insomnia-like phenotype and movement deficiency in zebrafish. Chemosphere. 253:126762.
Abstract
17β-trenbolone (17β-TBOH) is one of the dominant metabolites of trenbolone acetate, which is widely applied in beef cattle operations around the globe. The effects of environmental concentrations of 17β-trenbolone on the early development of zebrafish embryos have received very little attention. Melatonin could regulate sleep-wake cycle and plays a protective role in various adverse conditions. Here, environmentally realistic concentrations of 17β-trenbolone (1 ng/L, 10 ng/L, 50 ng/L) has been exposure to zebrafish embryos at 2 h postfertilization (hpf). The results showed that 10 ng/L and 50 ng/L 17β-trenbolone disturbed the distribution of caudal primary motoneurons and downregulated expression of motoneuron development related genes along with locomotion decreasing. While melatonin could recover the detrimental effects caused by 17β-trenbolone. Interestingly, 17β-trenbolone exposure increased waking activity and decreased rest even in a low dose (1 ng/L). Moreover, it upregulated hypocretin/orexin (Hcrt) signaling which promotes wakefulness. Melatonin restored the insomnia-like alternation induced by 17β-trenbolone exposure. Collectively, we conclude that 17β-trenbolone disturbed motoneuron development and altered sleep/wake behavior, while melatonin could alleviate the deleterious influence on motoneuron development and recover the circadian rhythm.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping