PUBLICATION
Application of Zebrafish Model in the Suppression of Drug-Induced Cardiac Hypertrophy by Traditional Indian Medicine Yogendra Ras
- Authors
- Balkrishna, A., Rustagi, Y., Bhattacharya, K., Varshney, A.
- ID
- ZDB-PUB-200422-129
- Date
- 2020
- Source
- Biomolecules 10(4): (Journal)
- Registered Authors
- Keywords
- Ayurveda, H9C2, Yogendra Ras, biomarkers, cardiac hypertrophy, erythromycin, isoproterenol, oxidative stress, zebrafish model
- MeSH Terms
-
- Animals
- Antioxidants/pharmacology
- Biomarkers/metabolism
- C-Reactive Protein/metabolism
- Cardiomegaly/blood
- Cardiomegaly/chemically induced*
- Cardiomegaly/drug therapy*
- Cardiomegaly/pathology
- Disease Models, Animal
- Erythromycin
- Heart Defects, Congenital/blood
- Heart Defects, Congenital/drug therapy
- Heart Defects, Congenital/pathology
- Isoproterenol
- Medicine, Ayurvedic*
- Oxidative Stress
- Phytotherapy*
- Platelet Aggregation
- Rats
- Spectrometry, X-Ray Emission
- X-Ray Diffraction
- Zebrafish/physiology*
- PubMed
- 32295034 Full text @ Biomolecules
Citation
Balkrishna, A., Rustagi, Y., Bhattacharya, K., Varshney, A. (2020) Application of Zebrafish Model in the Suppression of Drug-Induced Cardiac Hypertrophy by Traditional Indian Medicine Yogendra Ras. Biomolecules. 10(4):.
Abstract
Zebrafish is an elegant vertebrate employed to model the pathological etiologies of human maladies such as cardiac diseases. Persistent physiological stresses can induce abnormalities in heart functions such as cardiac hypertrophy (CH), which can lead to morbidity and mortality. In the present study, using zebrafish as a study model, efficacy of the traditional Indian Ayurveda medicine "Yogendra Ras" (YDR) was validated in ameliorating drug-induced cardiac hypertrophy. YDR was prepared using traditionally described methods and composed of nano- and micron-sized metal particles. Elemental composition analysis of YDR showed the presence of mainly Au, Sn, and Hg. Cardiac hypertrophy was induced in the zebrafish following a pretreatment with erythromycin (ERY), and the onset and reconciliation of disease by YDR were determined using a treadmill electrocardiogram, heart anatomy analysis, C-reactive protein release, and platelet aggregation time-analysis. YDR treatment of CH-induced zebrafish showed comparable results with the Standard-of-care drug, verapamil, tested in parallel. Under in-vitro conditions, treatment of isoproterenol (ISP)-stimulated murine cardiomyocytes (H9C2) with YDR resulted in the suppression of drug-stimulated biomarkers of oxidative stress: COX-2, NOX-2, NOX-4, ANF, troponin-I, -T, and cardiolipin. Taken together, zebrafish showed a strong disposition as a model for studying the efficacy of Ayurvedic medicines towards drug-induced cardiopathies. YDR provided strong evidence for its capability in modulating drug-induced CH through the restoration of redox homeostasis and exhibited potential as a viable complementary therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping