PUBLICATION
Conserved function of zebrafish (Danio rerio) Gdf15 as a sepsis tolerance mediator
- Authors
- Pereiro, P., Librán-Pérez, M., Figueras, A., Novoa, B.
- ID
- ZDB-PUB-200422-115
- Date
- 2020
- Source
- Developmental and comparative immunology 109: 103698 (Journal)
- Registered Authors
- Figueras, Antonio, Novoa, Beatriz
- Keywords
- Gdf15, LPS, Metabolism, Sepsis, Tolerance, Zebrafish
- MeSH Terms
-
- Aeromonas hydrophila/physiology
- Amino Acid Sequence
- Animals
- Base Sequence
- Disease Models, Animal*
- Gene Expression Profiling/methods
- Growth Differentiation Factor 15/classification
- Growth Differentiation Factor 15/genetics*
- Growth Differentiation Factor 15/metabolism
- Host-Pathogen Interactions
- Humans
- Larva/genetics
- Larva/metabolism
- Larva/microbiology
- Lipopolysaccharides
- Mice
- Phylogeny
- Sepsis/chemically induced
- Sepsis/genetics*
- Sepsis/microbiology
- Survival Analysis
- Zebrafish Proteins/classification
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 32289326 Full text @ Dev. Comp. Immunol.
Citation
Pereiro, P., Librán-Pérez, M., Figueras, A., Novoa, B. (2020) Conserved function of zebrafish (Danio rerio) Gdf15 as a sepsis tolerance mediator. Developmental and comparative immunology. 109:103698.
Abstract
GDF15 is frequently detected in patients suffering from various diseases, especially those associated with pro-inflammatory processes and/or metabolic disorders. Accordingly, sepsis, whose major complications are related to metabolic alterations and systemic inflammation, significantly increases the secretion of GDF15. Indeed, this cytokine could be considered a marker of sepsis severity. However, until the last several years, the involvement of GDF15 in these disorders had not been widely characterized. In mice, GDF15 was recently described as a pivotal inducer of sepsis tolerance by mediating metabolic alterations that reduce tissue damage. In this work we describe a zebrafish gdf15 gene. We found that gdf15 follows an expression pattern similar to that observed in mammals, being highly expressed in the liver and kidney and induced after pro-inflammatory stimuli. Moreover, larvae overexpressing gdf15 were more resistant to bacterial and viral challenges without affecting the pathogen load. Consequently, Gdf15 also protected zebrafish larvae against LPS-induced mortality. As in mice, zebrafish Gdf15 seems to induce sepsis tolerance by altering the metabolic parameters of the individuals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping