PUBLICATION
Phoenixin-20 Stimulates mRNAs Encoding Hypothalamo-Pituitary-Gonadal Hormones, is Pro-Vitellogenic, and Promotes Oocyte Maturation in Zebrafish
- Authors
- Rajeswari, J.J., Unniappan, S.
- ID
- ZDB-PUB-200422-111
- Date
- 2020
- Source
- Scientific Reports 10: 6264 (Journal)
- Registered Authors
- Unniappan, Suraj
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line
- Female
- Gonadotropin-Releasing Hormone/metabolism
- Gonads/drug effects
- Hepatocytes/cytology
- Hepatocytes/drug effects*
- Kisspeptins/metabolism
- Male
- Oocytes/cytology
- Oocytes/drug effects*
- Peptide Hormones/pharmacology*
- RNA, Messenger/metabolism
- Vitellogenesis/drug effects*
- Vitellogenins/metabolism
- Zebrafish
- PubMed
- 32286445 Full text @ Sci. Rep.
Citation
Rajeswari, J.J., Unniappan, S. (2020) Phoenixin-20 Stimulates mRNAs Encoding Hypothalamo-Pituitary-Gonadal Hormones, is Pro-Vitellogenic, and Promotes Oocyte Maturation in Zebrafish. Scientific Reports. 10:6264.
Abstract
Phoenixin-20 (PNX-20) is a bioactive peptide with hormone-like actions in vertebrates. In mammals, PNX stimulates hypothalamo-pituitary-gonadal hormones and regulate reproductive processes. Our immunohisto/cytochemical studies show PNX-like and the putative PNX receptor, SREB3-like immunoreactivity in the gonads of zebrafish, and in zebrafish liver (ZFL) cells. Intraperitoneal injection of zebrafish PNX-20 upregulates mRNAs encoding both salmon gonadotropin-releasing hormone (GnRH), and chicken GnRH-II and kisspeptin and its receptor in zebrafish hypothalamus. Similarly, luteinizing hormone receptor mRNA expression in the testis, follicle-stimulating hormone receptor in the ovary, and the kisspeptin system were upregulated in the gonads of PNX-20 injected fish. We also observed the upregulation of genes involved in the sex steroidogenic pathway (cyp11a1, cyp17a1, 17βhsd, cyp19a1a) in the gonads of PNX-20 administered fish. PNX-20 upregulates the expression of vitellogenin isoforms and estrogen receptor (esr2a and 2b) mRNAs in ZFL cells in vitro. Meanwhile, siRNA-mediated knockdown of PNX-20 resulted in the downregulation of all vitellogenin transcripts, further suggesting its possible role in vitellogenesis. PNX-20 treatment resulted in a significant increase in germinal vesicle breakdown in zebrafish follicles in vitro. Collectively, these results provide strong evidence for PNX-20 effects on the HPG axis and liver to promote reproduction in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping