PUBLICATION
Spindle-F-actin interactions in mitotic spindles in an intact vertebrate epithelium
- Authors
- Kita, A.M., Swider, Z.T., Erofeev, I., Halloran, M.C., Goryachev, A.B., Bement, W.M.
- ID
- ZDB-PUB-200422-1
- Date
- 2019
- Source
- Molecular biology of the cell 30: 1645-1654 (Journal)
- Registered Authors
- Halloran, Mary
- Keywords
- none
- MeSH Terms
-
- Actins/metabolism*
- Animals
- Cell Survival
- Endoplasmic Reticulum/metabolism
- Epithelial Cells/metabolism
- Epithelium/metabolism*
- Formins/metabolism
- Spindle Apparatus/metabolism*
- Spindle Poles/metabolism
- Xenopus laevis/metabolism*
- PubMed
- 31091161 Full text @ Mol. Biol. Cell
Citation
Kita, A.M., Swider, Z.T., Erofeev, I., Halloran, M.C., Goryachev, A.B., Bement, W.M. (2019) Spindle-F-actin interactions in mitotic spindles in an intact vertebrate epithelium. Molecular biology of the cell. 30:1645-1654.
Abstract
Mitotic spindles are well known to be assembled from and dependent on microtubules. In contrast, whether actin filaments (F-actin) are required for or are even present in mitotic spindles has long been controversial. Here we have developed improved methods for simultaneously preserving F-actin and microtubules in fixed samples and exploited them to demonstrate that F-actin is indeed associated with mitotic spindles in intact Xenopus laevis embryonic epithelia. We also find that there is an "F-actin cycle," in which the distribution and organization of spindle F-actin changes over the course of the cell cycle. Live imaging using a probe for F-actin reveals that at least two pools of F-actin are associated with mitotic spindles: a relatively stable internal network of cables that moves in concert with and appears to be linked to spindles, and F-actin "fingers" that rapidly extend from the cell cortex toward the spindle and make transient contact with the spindle poles. We conclude that there is a robust endoplasmic F-actin network in normal vertebrate epithelial cells and that this network is also a component of mitotic spindles. More broadly, we conclude that there is far more internal F-actin in epithelial cells than is commonly believed.
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