PUBLICATION
Proteomic Profiling of Zebrafish Challenged by Spring Viremia of Carp Virus Provides Insight into Skin Antiviral Response
- Authors
- Liu, R., Hu, X., Lü, A., Song, Y., Lian, Z., Sun, J., Sung, Y.Y.
- ID
- ZDB-PUB-200403-85
- Date
- 2020
- Source
- Zebrafish 17: 91-103 (Journal)
- Registered Authors
- Keywords
- SVCV, immune response, proteomics, skin, zebrafish
- MeSH Terms
-
- Animals
- Fish Diseases/immunology*
- Fish Diseases/virology
- Fish Proteins/immunology*
- Proteome/immunology*
- Rhabdoviridae/physiology*
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary*
- Rhabdoviridae Infections/virology
- Skin/immunology*
- Zebrafish/immunology*
- PubMed
- 32176570 Full text @ Zebrafish
Citation
Liu, R., Hu, X., Lü, A., Song, Y., Lian, Z., Sun, J., Sung, Y.Y. (2020) Proteomic Profiling of Zebrafish Challenged by Spring Viremia of Carp Virus Provides Insight into Skin Antiviral Response. Zebrafish. 17:91-103.
Abstract
Spring viremia of carp virus (SVCV) causes the skin hemorrhagic disease in cyprinid species, but its molecular mechanism of skin immune response remains unclear at the protein level. In the present study, the differential proteomics of the zebrafish (Danio rerio) skin in response to SVCV infection were examined by isobaric tags for relative and absolute quantitation and quantitative polymerase chain reaction (qPCR) assays. A total of 3999 proteins were identified, of which 320 and 181 proteins were differentially expressed at 24 and 96 h postinfection, respectively. The expression levels of 16 selected immune-related differentially expressed proteins (DEPs) were confirmed by qPCR analysis. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that DEPs were significantly associated with complement, inflammation, and antiviral response. The protein-protein interaction network of cytoskeleton-associated proteins, ATPase-related proteins, and parvalbumins from DEPs was shown to be involved in skin immune response. This is first report on the skin proteome profiling of zebrafish against SVCV infection, which will contribute to understand the molecular mechanism of local mucosal immunity in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping