PUBLICATION
Pro-angiogenic activity of Tongnao decoction on HUVECs in vitro and zebrafish in vivo
- Authors
- Wang, J., Zhang, X.H., Xu, X., Zhu, Q., Yao, B., Liang, S., Chen, Z., Wang, Y., He, M.F., Wu, M.
- ID
- ZDB-PUB-200403-7
- Date
- 2020
- Source
- Journal of ethnopharmacology 254: 112737 (Journal)
- Registered Authors
- Keywords
- Chinese herbal decoction, HUVECs, Inhibitors, Pro-angiogenic activity, Tongnao decoction, Zebrafish
- MeSH Terms
-
- Angiogenesis Inducing Agents/pharmacology*
- Animals
- Animals, Genetically Modified
- Blood Vessels/drug effects
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Cell Survival/drug effects
- Cells, Cultured
- Embryo, Nonmammalian/blood supply
- Human Umbilical Vein Endothelial Cells/drug effects
- Human Umbilical Vein Endothelial Cells/physiology
- Humans
- Wound Healing/drug effects
- Zebrafish
- PubMed
- 32147480 Full text @ J. Ethnopharmacol.
Citation
Wang, J., Zhang, X.H., Xu, X., Zhu, Q., Yao, B., Liang, S., Chen, Z., Wang, Y., He, M.F., Wu, M. (2020) Pro-angiogenic activity of Tongnao decoction on HUVECs in vitro and zebrafish in vivo. Journal of ethnopharmacology. 254:112737.
Abstract
Ethnopharmacological relevance Tongnao Decoction (TND) is a Chinese decoction approved and used in Jiangsu Province Hospital for the treatment of ischemic stroke. It shows conclusive efficiency in the improvement of neurologic impairment and activities of daily living of the patients.
Aim of the study Recently, angiogenesis has been recognized as a potential therapeutic strategy for treating cerebral ischemia. This study was aimed to provide comprehensive evidence for the pro-angiogenic effect of TND and characterize the underlying mechanism.
Materials and methods We firstly established the chemical fingerprinting of TND. Then, the in vitro pro-angiogenic activities of TND were tested on human umbilical vein endothelial cells (HUVECs) through cell viability, wound healing and tube formation assays. The in vivo pro-angiogenic effects were evaluated on transgenic zebrafish embryos [Tg (fli-1: EGFP)] through the formation of intersegmental vessels (ISVs), subintestinal vessels (SIVs) and central arteries (CtAs). Lastly, the potential mechanisms of TND were analyzed by a blocking assay with eight pathways-specific kinase inhibitors.
Results TND promoted the proliferation, migration and tube formation of HUVECs. TND also rescued the impairment of ISVs, SIVs and CtAs caused by VRI in a dose-dependent manner in zebrafish embryos. TND could activate vascular endothelial growth factor receptor-2 (VEGFR-2), phosphoinositide 3-kinase (PI3K) - protein kinase B (Akt) and Raf - mitogen-activated protein kinase1/2 (MEK1/2) - extracellular regulated kinase 1/2 (ERK1/2) signaling pathways.
Conclusion Our study firstly demonstrated the pro-angiogenic activities of TND. Our work provided evidences for the clinical usage of TND in restoring neurovascular function through promoting angiogenesis in the ischemic cerebral microvascular.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping