PUBLICATION

Host monitoring of quorum sensing during Pseudomonas aeruginosa infection

Authors

Moura-Alves, P., Puyskens, A., Stinn, A., Klemm, M., Guhlich-Bornhof, U., Dorhoi, A., Furkert, J., Kreuchwig, A., Protze, J., Lozza, L., Pei, G., Saikali, P., Perdomo, C., Mollenkopf, H.J., Hurwitz, R., Kirschhoefer, F., Brenner-Weiss, G., Weiner, J., Oschkinat, H., Kolbe, M., Krause, G., Kaufmann, S.H.E.

ID

ZDB-PUB-200403-256

Date

2019

Source

Science (New York, N.Y.) 366(6472): (Journal)

Registered Authors

Keywords

none

Datasets

GEO:GSE121101

MeSH Terms

A549 Cells
Animals
Mice, Knockout
Zebrafish
Pseudomonas aeruginosa/genetics
Pseudomonas aeruginosa/pathogenicity*
Pseudomonas Infections/microbiology*
Macrophages/microbiology
Receptors, Aryl Hydrocarbon/genetics
Receptors, Aryl Hydrocarbon/physiology*
Host-Pathogen Interactions*
Humans
Mice
Larva
Quorum Sensing/genetics
Quorum Sensing/physiology*

(all 16)

PubMed

31857448 Full text @ Science

Abstract

Pseudomonas aeruginosa rapidly adapts to altered conditions by quorum sensing (QS), a communication system that it uses to collectively modify its behavior through the production, release, and detection of signaling molecules. QS molecules can also be sensed by hosts, although the respective receptors and signaling pathways are poorly understood. We describe a pattern of regulation in the host by the aryl hydrocarbon receptor (AhR) that is critically dependent on qualitative and quantitative sensing of P. aeruginosa quorum. QS molecules bind to AhR and distinctly modulate its activity. This is mirrored upon infection with P. aeruginosa collected from diverse growth stages and with QS mutants. We propose that by spying on bacterial quorum, AhR acts as a major sensor of infection dynamics, capable of orchestrating host defense according to the status quo of infection.

Genes / Markers

Figures

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Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
AB	chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine	Long-pec	whole organism	RTPCR	Fig. S7
AB	chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine	Long-pec	whole organism	RTPCR	Fig. S7
AB	bacterial treatment by exposure to environment: Pseudomonas aeruginosa PA14	Long-pec	whole organism	RTPCR	Fig. 4
AB	chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine	Long-pec	whole organism	RTPCR	Fig. S7

1 - 4 of 4

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB	chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine	Long-pec	whole organism ahrra expression increased amount, abnormal	Fig. S7
AB	chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine	Long-pec	whole organism ahrrb expression increased amount, abnormal	Fig. S7
AB	chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine	Long-pec	whole organism cyp1a expression increased amount, abnormal	Fig. S7
AB	bacterial treatment by exposure to environment: Pseudomonas aeruginosa PA14	Long-pec	whole organism cyp1a expression increased amount, abnormal	Fig. 4

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Mutations / Transgenics

Human Disease / Model

Human Disease	Fish	Conditions	Evidence
bacterial infectious disease	AB	bacterial treatment by exposure to environment: Pseudomonas aeruginosa PA14	TAS

Sequence Targeting Reagents

Fish

Fish
AB

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Moura-Alves et al., 2019 ZDB-PUB-200403-256 PMID:31857448

Host monitoring of quorum sensing during Pseudomonas aeruginosa infection

Moura-Alves et al., 2019

ZDB-PUB-200403-256
PMID:31857448