PUBLICATION
Phoenixin-20 (PNX-20) Suppresses Food Intake, Modulates Glucoregulatory Enzymes, and Enhances Glycolysis in Zebrafish
- Authors
- Rajeswari, J.J., Blanco, A.M., Unniappan, S.
- ID
- ZDB-PUB-200403-174
- Date
- 2020
- Source
- American journal of physiology. Regulatory, integrative and comparative physiology 318(5): R917-R928 (Journal)
- Registered Authors
- Unniappan, Suraj
- Keywords
- Food Intake, Glucose homeostasis, Metabolism, Phoenixin, Zebrafish
- MeSH Terms
-
- Animals
- Appetite Depressants/pharmacology*
- Appetite Regulation/drug effects
- Cell Line
- Eating/drug effects*
- Feeding Behavior/drug effects*
- Female
- Gene Expression Regulation
- Glucose/metabolism*
- Glycolysis/drug effects*
- Glycolysis/genetics
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Homeodomain Proteins/pharmacology*
- Male
- Peptide Fragments/genetics
- Peptide Fragments/metabolism
- Peptide Fragments/pharmacology*
- Signal Transduction
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/pharmacology*
- PubMed
- 32208925 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Citation
Rajeswari, J.J., Blanco, A.M., Unniappan, S. (2020) Phoenixin-20 (PNX-20) Suppresses Food Intake, Modulates Glucoregulatory Enzymes, and Enhances Glycolysis in Zebrafish. American journal of physiology. Regulatory, integrative and comparative physiology. 318(5):R917-R928.
Abstract
Phoenixin is a 20 amino acid peptide (PNX-20) cleaved from the small integral membrane protein 20 (SMIM20), with multiple biological roles in mammals. However, its role in non-mammalian vertebrates is poorly understood. This research aimed to determine whether PNX-20 influences feeding and metabolism in zebrafish. The mRNAs encoding SMIM20 and its putative receptor, super conserved receptor expressed in brain 3 (SREB3), are present in both central and peripheral tissues of zebrafish. Immunohistochemical analysis confirmed the presence of PNX-like immunoreactivity in the gut and zebrafish liver (ZFL) cell line. We also found that short-term fasting (7 days) significantly decreased smim20 mRNA expression in the brain, gut, liver, gonad, and muscle, which suggests a role for PNX-20 in food intake regulation. Indeed, single intraperitoneal injection of 1000 ng/g body weight PNX-20 reduced feeding in both male and female zebrafish, likely in part by enhancing hypothalamic cart and reducing hypothalamic/gut preproghrelin mRNAs. Furthermore, present results demonstrated that PNX-20 modulates the expression of genes involved in glucose transport and metabolism in ZFL cells. In general terms, such PNX-induced modulation of gene expression was characterized by the upregulation of glycolytic genes and the downregulation of gluconeogenic genes. A kinetic study of the ATP production rate from both glycolytic and mitochondrial pathways demonstrated that PNX-20-treated ZFL cells exhibited significantly higher ATP production rate associated to glycolysis than control cells, confirming a positive role for PNX-20 on glycolysis. Together, these results point out that PNX-20 is an anorexigen with important metabolic roles in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping