PUBLICATION
Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish
- Authors
- Ren, F., Lin, Q., Gong, G., Du, X., Dan, H., Qin, W., Miao, R., Xiong, Y., Xiao, R., Li, X., Gui, J.F., Mei, J.
- ID
- ZDB-PUB-200305-5
- Date
- 2020
- Source
- Communications biology 3: 94 (Journal)
- Registered Authors
- Gui, Jian-Fang, Li, Xiaohui, Mei, Jie
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian
- Embryonic Development/genetics*
- Gene Expression Regulation, Developmental
- RNA Stability/genetics
- RNA, Messenger, Stored/genetics
- RNA, Messenger, Stored/metabolism*
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/physiology*
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Zygote/metabolism
- PubMed
- 32127635 Full text @ Commun Biol
Citation
Ren, F., Lin, Q., Gong, G., Du, X., Dan, H., Qin, W., Miao, R., Xiong, Y., Xiao, R., Li, X., Gui, J.F., Mei, J. (2020) Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish. Communications biology. 3:94.
Abstract
Early embryogenesis relies on maternally inherited mRNAs. Although the mechanism of maternal mRNA degradation during maternal-to-zygotic transition (MZT) has been extensively studied in vertebrates, how the embryos maintain maternal mRNA stability remains unclear. Here, we identify Igf2bp3 as an important regulator of maternal mRNA stability in zebrafish. Depletion of maternal igf2bp3 destabilizes maternal mRNAs prior to MZT and leads to severe developmental defects, including abnormal cytoskeleton organization and cell division. However, the process of oogenesis and the expression levels of maternal mRNAs in unfertilized eggs are normal in maternal igf2bp3 mutants. Gene ontology analysis revealed that these functions are largely mediated by Igf2bp3-bound mRNAs. Indeed, Igf2bp3 depletion destabilizes while its overexpression enhances its targeting maternal mRNAs. Interestingly, igf2bp3 overexpression in wild-type embryos also causes a developmental delay. Altogether, these findings highlight an important function of Igf2bp3 in controlling early zebrafish embryogenesis by binding and regulating the stability of maternal mRNAs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping