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ZFIN ID: ZDB-PUB-200305-11
Functional Diversity of Glycinergic Commissural Inhibitory Neurons in Larval Zebrafish
Satou, C., Sugioka, T., Uemura, Y., Shimazaki, T., Zmarz, P., Kimura, Y., Higashijima, S.I.
Date: 2020
Source: Cell Reports   30: 3036-3050.e4 (Journal)
Registered Authors: Higashijima, Shin-ichi
Keywords: V0d neurons, commissural inhibitory neurons, dI6 dmrt3a neurons, dbx1, glycinergic interneuons, mid cycle inhibition, spinal cord circuit, zebrafish
MeSH Terms:
  • Animals
  • Gene Deletion
  • Glycine/metabolism*
  • Green Fluorescent Proteins/metabolism
  • Inhibitory Postsynaptic Potentials
  • Larva
  • Locomotion
  • Neural Inhibition/physiology*
  • Neurons/physiology*
  • Phenotype
  • Spinal Cord/physiology
  • Swimming
  • Transcription Factors/metabolism
  • Zebrafish/physiology*
  • Zebrafish Proteins/metabolism
PubMed: 32130905 Full text @ Cell Rep.
ABSTRACT
Commissural inhibitory neurons in the spinal cord of aquatic vertebrates coordinate left-right body alternation during swimming. Their developmental origin, however, has been elusive. We investigate this by comparing the anatomy and function of two commissural inhibitory neuron types, dI6dmrt3a and V0d, derived from the pd6 and p0 progenitor domains, respectively. We find that both of these commissural neuron types have monosynaptic, inhibitory connections to neuronal populations active during fictive swimming, supporting their role in providing inhibition to the contralateral side. V0d neurons tend to fire during faster and stronger movements, while dI6dmrt3a neurons tend to fire more consistently during normal fictive swimming. Ablation of dI6dmrt3a neurons leads to an impairment of left-right alternating activity through abnormal co-activation of ventral root neurons on both sides of the spinal cord. Our results suggest that dI6dmrt3a and V0d commissural inhibitory neurons synergistically provide inhibition to the opposite side across different swimming behaviors.
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