PUBLICATION

Influence of dietary iron exposure on trace metal homeostasis and expression of metal transporters during development in zebrafish☆

Authors
Chandrapalan, T., Kwong, R.W.M.
ID
ZDB-PUB-200301-6
Date
2020
Source
Environmental pollution (Barking, Essex : 1987)   261: 114159 (Journal)
Registered Authors
Keywords
Development, Dietary exposure, Iron, Metal homeostasis, Zebrafish
MeSH Terms
  • Animals
  • Homeostasis
  • Iron
  • Iron, Dietary*
  • Zebrafish
  • Zebrafish Proteins*
PubMed
32113105 Full text @ Environ. Pollut.
Citation
Chandrapalan, T., Kwong, R.W.M. (2020) Influence of dietary iron exposure on trace metal homeostasis and expression of metal transporters during development in zebrafish☆. Environmental pollution (Barking, Essex : 1987). 261:114159.
Abstract
The present study investigated the effects of dietary iron (Fe) exposure on physiological performance and homeostatic regulation of trace metals during development (5-28 days post-fertilization; dpf) in zebrafish (Danio rerio). The results demonstrated that whole body Fe content was increased in 14 dpf larvae fed a high Fe diet. Cumulative mortality was also significantly elevated during exposure to the high Fe diet. Using droplet digital PCR, we observed that high Fe-exposed larvae exhibited an increase in mRNA levels of the Fe-storage protein ferritin, which appeared to be associated with the elevated level of whole body Fe content. Further, the results indicated that dietary Fe exposure induced transient changes in the mRNA expression levels of various metal transporters, including the iron transporter dmt1, and the zinc transporters zip8 and zip14. The expression of the epithelial Ca2+ channels (i.e., ecac) was also found to increase by high dietary Fe. Overall, our findings suggest that larval fish during the early nutritional transition period are sensitive to the effects of dietary Fe.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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