PUBLICATION
Epithelial Planar Bipolarity Emerges from Notch-Mediated Asymmetric Inhibition of Emx2
- Authors
- Kozak, E.L., Palit, S., Miranda-Rodríguez, J.R., Janjic, A., Böttcher, A., Lickert, H., Enard, W., Theis, F.J., López-Schier, H.
- ID
- ZDB-PUB-200229-17
- Date
- 2020
- Source
- Current biology : CB 30(6): 1142-1151.e6 (Journal)
- Registered Authors
- Lopez-Schier, Hernan
- Keywords
- diffusion pseudotime, hair cells, planar polarity, single-cell RNA sequencing
- Datasets
- GEO:GSE144827, GEO:GSE143663
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/embryology*
- Gene Expression Regulation
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism
- Lateral Line System/embryology*
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/metabolism
- Receptor, Notch1/genetics*
- Receptor, Notch1/metabolism
- Signal Transduction
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 32109392 Full text @ Curr. Biol.
Citation
Kozak, E.L., Palit, S., Miranda-Rodríguez, J.R., Janjic, A., Böttcher, A., Lickert, H., Enard, W., Theis, F.J., López-Schier, H. (2020) Epithelial Planar Bipolarity Emerges from Notch-Mediated Asymmetric Inhibition of Emx2. Current biology : CB. 30(6):1142-1151.e6.
Abstract
Most plane-polarized tissues are formed by identically oriented cells [1, 2]. A notable exception occurs in the vertebrate vestibular system and lateral-line neuromasts, where mechanosensory hair cells orient along a single axis but in opposite directions to generate bipolar epithelia [3-5]. In zebrafish neuromasts, pairs of hair cells arise from the division of a non-sensory progenitor [6, 7] and acquire opposing planar polarity via the asymmetric expression of the polarity-determinant transcription factor Emx2 [8-11]. Here, we reveal the initial symmetry-breaking step by decrypting the developmental trajectory of hair cells using single-cell RNA sequencing (scRNA-seq), diffusion pseudotime analysis, lineage tracing, and mutagenesis. We show that Emx2 is absent in non-sensory epithelial cells, begins expression in hair-cell progenitors, and is downregulated in one of the sibling hair cells via signaling through the Notch1a receptor. Analysis of Emx2-deficient specimens, in which every hair cell adopts an identical direction, indicates that Emx2 asymmetry does not result from auto-regulatory feedback. These data reveal a two-tiered mechanism by which the symmetric monodirectional ground state of the epithelium is inverted by deterministic initiation of Emx2 expression in hair-cell progenitors and a subsequent stochastic repression of Emx2 in one of the sibling hair cells breaks directional symmetry to establish planar bipolarity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping