PUBLICATION

Selective detection of pyrophosphate anion by zinc ensemble of C3-symmetric triaminoguanidine-pyrrole conjugate and its biosensing applications

Authors
Tharmalingam, B., Mathivanan, M., Mani, K.S., Kaminsky, W., Raghunath, A., Jothi, M., Perumal, E., Murugesapandian, B.
ID
ZDB-PUB-200225-7
Date
2020
Source
Analytica chimica acta   1103: 192-201 (Journal)
Registered Authors
Azhwar, Raghunath
Keywords
INHIBIT logic gate, PCR products, PPi detection, Triaminoguanidine-pyrrole conjugate, Turn-on sensor, Zebrafish
MeSH Terms
  • Animals
  • Biosensing Techniques
  • Colorimetry/instrumentation
  • Diphosphates/analysis*
  • Fluorescent Dyes/chemistry*
  • Guanidines/chemistry*
  • Guanidines/toxicity
  • Hydrogen-Ion Concentration
  • Limit of Detection
  • Logic
  • Microscopy, Fluorescence
  • Paper
  • Pyrroles/chemistry*
  • Pyrroles/toxicity
  • Spectrometry, Fluorescence
  • Zebrafish
  • Zinc/chemistry*
PubMed
32081184 Full text @ Anal. Chim. Acta
Citation
Tharmalingam, B., Mathivanan, M., Mani, K.S., Kaminsky, W., Raghunath, A., Jothi, M., Perumal, E., Murugesapandian, B. (2020) Selective detection of pyrophosphate anion by zinc ensemble of C3-symmetric triaminoguanidine-pyrrole conjugate and its biosensing applications. Analytica chimica acta. 1103:192-201.
Abstract
A new-fangled C3-symmetric triaminoguanidine-pyrrole conjugate has been constructed and utilized for sensing applications. The probe selectively detects zinc ions (Zn2+) by colorimetric as well as turn-on fluorescent manner. Further, the in-situ formed zinc ensemble displays turn-off fluorescence response towards the pyrophosphate anion (PPi) via displacement approach. Emissive off-on-off sensing characteristics of the probe has been successfully exploited to construct the INHIBIT logic gate, coding/decoding of messages and in vivo imaging of Zn2+/PPi in zebrafish larvae. Further, PPi detection characteristics of zinc ensembles were established for the sensing of PPi discharged from DNA synthesis and other biological reactions.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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