PUBLICATION
A natural complex product Yaocha reduces uric acid level in a live zebrafish model
- Authors
- Xiong, X.Y., Liang, J., Guo, S.Y., Dai, M.Z., Zhou, J.L., Zhang, Y., Liu, Y.
- ID
- ZDB-PUB-200225-31
- Date
- 2020
- Source
- Journal of Pharmacological and Toxicological Methods 102: 106681 (Journal)
- Registered Authors
- Keywords
- High uric acid, Hyperuricemia therapeutics, Uricase inhibitor, Yaocha, Zebrafish
- MeSH Terms
-
- Animals
- Aspalathus/chemistry
- Biological Products/administration & dosage
- Biological Products/chemistry
- Biological Products/pharmacology*
- Dipeptides/administration & dosage
- Dipeptides/pharmacology
- Disease Models, Animal
- Hyperuricemia/drug therapy*
- Hypoxanthine Phosphoribosyltransferase/genetics
- Organic Anion Transport Protein 1/genetics
- Theaceae/chemistry
- Uric Acid/blood*
- Zebrafish
- PubMed
- 32087362 Full text @ J. Pharmacol. Toxicol. Methods
Citation
Xiong, X.Y., Liang, J., Guo, S.Y., Dai, M.Z., Zhou, J.L., Zhang, Y., Liu, Y. (2020) A natural complex product Yaocha reduces uric acid level in a live zebrafish model. Journal of Pharmacological and Toxicological Methods. 102:106681.
Abstract
Introduction This study was aimed to assess uric acid (UA)-lowering effect and its possible mechanisms of a natural complex product Yaocha in a live zebrafish model.
Methods The zebrafish high UA model was established by feeding 5 dpf zebrafish with both an uricase inhibitor potassium oxonate at 10 mM and an UA synthesis precursor xanthine sodium at 0.5 mM for 24 h.. Yaocha was administered to the high UA zebrafish through soaking at 3 various concentrations, with allopurinol as a positive control. UA level, xanthine oxidase (XOD) activity, and mRNA expression of hypoxanthine guanine-phosphoribosyltransferases transferase (HPRT1) and organic anion transporter 1 (OAT1) were measured.
Results Yaocha effectively reduced UA level and inhibited xanthine oxidase (XO) activity in the high UA zebrafish. Yaocha could be a potential therapeutics for hyperuricemia through up-regulating HPRT1 and OAT1 gene expression and suppressing XO activity.
Discussion These results suggested that Yaocha hold a potential for high UA prevention and therapy, possibly through inhibiting UA production and promoting urate secretion and purine conversion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping