PUBLICATION
Molecular and functional characterization of tilapia DDX41 in IFN regulation
- Authors
- Gan, Z., Cheng, J., Hou, J., Xia, H., Chen, W., Xia, L., Nie, P., Lu, Y.
- ID
- ZDB-PUB-200225-10
- Date
- 2020
- Source
- Fish & shellfish immunology 99: 386-391 (Journal)
- Registered Authors
- Nie, Pin
- Keywords
- Cytosolic DNA sensors, DDX41, Oreochromis niloticus, Promoter activity, Type I interferon
- MeSH Terms
-
- Interferons/genetics
- Interferons/metabolism*
- Gene Expression Regulation, Enzymologic/immunology*
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- PubMed
- 32081808 Full text @ Fish Shellfish Immunol.
Abstract
DEAD-box helicase 41 (DDX41) is a key cytosolic DNA sensor playing critical roles in the regulation of type I IFN responses, and their functions have been well-characterized in mammals. However, little information is available regarding the function of fish DDX41. In this study, a DDX41 gene, named On-DDX41, was identified in Nile tilapia, Oreochromis niloticus. The predicted protein of On-DDX41 contains several structural features known in DDX41, including conserved DEADc and HELICc domains, and a conserved sequence "Asp-Glu-Ala-Asp (D-E-A-D)". On-DDX41 gene was constitutively expressed in all tissues examined, with the highest expression level observed in liver and muscle, and was inducible after poly(I:C) stimulation. Moreover, the overexpression of On-DDX41 can elicit a strong activation of both zebrafish IFN1 and IFN3 promoter in fish cells treated with poly(dA:dT). The present study thus contributes to a better understanding of the functional properties of DDX41 in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping