ZFIN ID: ZDB-PUB-200216-12
High-contrast, synchronous volumetric imaging with selective volume illumination microscopy
Truong, T.V., Holland, D.B., Madaan, S., Andreev, A., Keomanee-Dizon, K., Troll, J.V., Koo, D.E.S., McFall-Ngai, M.J., Fraser, S.E.
Date: 2020
Source: Communications biology   3: 74 (Journal)
Registered Authors: Fraser, Scott E.
Keywords: none
MeSH Terms:
  • Animals
  • Brain/anatomy & histology
  • Brain/diagnostic imaging
  • Brain/ultrastructure
  • Decapodiformes/microbiology
  • Decapodiformes/ultrastructure
  • Heart/anatomy & histology
  • Heart/diagnostic imaging
  • Heart/physiology
  • Host Microbial Interactions/physiology
  • Image Processing, Computer-Assisted/instrumentation
  • Image Processing, Computer-Assisted/methods*
  • Imaging, Three-Dimensional/instrumentation
  • Imaging, Three-Dimensional/methods*
  • Larva
  • Light
  • Microscopy, Fluorescence/instrumentation
  • Microscopy, Fluorescence/methods
  • Organ Size
  • Seawater/microbiology
  • Video Recording/instrumentation
  • Video Recording/methods
  • Zebrafish
PubMed: 32060411 Full text @ Commun Biol
Light-field fluorescence microscopy uniquely provides fast, synchronous volumetric imaging by capturing an extended volume in one snapshot, but often suffers from low contrast due to the background signal generated by its wide-field illumination strategy. We implemented light-field-based selective volume illumination microscopy (SVIM), where illumination is confined to only the volume of interest, removing the background generated from the extraneous sample volume, and dramatically enhancing the image contrast. We demonstrate the capabilities of SVIM by capturing cellular-resolution 3D movies of flowing bacteria in seawater as they colonize their squid symbiotic partner, as well as of the beating heart and brain-wide neural activity in larval zebrafish. These applications demonstrate the breadth of imaging applications that we envision SVIM will enable, in capturing tissue-scale 3D dynamic biological systems at single-cell resolution, fast volumetric rates, and high contrast to reveal the underlying biology.